ST131 fimH22 Escherichia coli isolate with a blaCMY-2/IncI1/ST12 plasmid obtained from a patient with bloodstream infection: highly similar to E. coli isolates of broiler origin

Louise Roer, Søren Overballe-Petersen, Frank Hansen, Thor B Johannesen, Marc Stegger, Valeria Bortolaia, Pimlapas Leekitcharoenphon, Helle Bisgaard Korsgaard, Anne Mette Seyfarth, Joël Mossong, Pierre Wattiau, Cécile Boland, Dennis S Hansen, Henrik Hasman, Anette M Hammerum*, Rene S. Hendriksen

*Corresponding author for this work

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

Objectives: This study compares the genome of an ST131 CMY-2-producing Escherichia coli isolate from a Danish patient with other ST131 CMY-2-producing E. coli isolates of both human and animal origin.

Methods: In 2016, an ST131 CMY-2-producing E. coli isolate (ESBL20160056) was obtained from a patient with a bloodstream infection. The genome of the ESBL20160056 isolate was compared with genomes from six ST131 CMY-2-producing E. coli isolates obtained from broiler meat imported to Denmark, 15 ST131 CMY-2-producing E. coli isolates obtained from Enterobase (http://enterobase.warwick.ac.uk) and two ST131 CMY-2-producing E. coli from European collaborators. The plasmid from ESBL20160056 was sequenced using a MinION Mk1B (Oxford Nanopore Technologies).

Results: The E. coli isolate from the Danish patient clustered together with 13 other fimH22 ST131 CMY-2-producing E. coli isolates in a distinct clade. The clade consisted of genomes from six E. coli isolates from humans collected in Denmark, Spain, Cambodia and the USA, six E. coli isolates obtained from broiler meat samples imported to Denmark from France, the Netherlands and Germany, and two E. coli isolates obtained from broilers in Belgium and Luxembourg. The 101.5 kb plasmid with blaCMY-2 from ESBL20160056 had an IncI1 replicon and belonged to ST12 using the plasmid MLST scheme. In total, 10 of the 14 ST131 E. coli isolates belonging to the fimH22 clade carried an IncI1 ST12 plasmid with blaCMY-2.

Conclusions: From our data, it seems plausible that the ST131 fimH22 CMY-2-producing E. coli isolate obtained from the Danish patient could have a zoonotic broiler origin.

Original languageEnglish
JournalJournal of Antimicrobial Chemotherapy
Volume74
Issue number3
Pages (from-to)557-560
ISSN0305-7453
DOIs
Publication statusPublished - 2019

Cite this

Roer, Louise ; Overballe-Petersen, Søren ; Hansen, Frank ; Johannesen, Thor B ; Stegger, Marc ; Bortolaia, Valeria ; Leekitcharoenphon, Pimlapas ; Bisgaard Korsgaard, Helle ; Seyfarth, Anne Mette ; Mossong, Joël ; Wattiau, Pierre ; Boland, Cécile ; Hansen, Dennis S ; Hasman, Henrik ; Hammerum, Anette M ; Hendriksen, Rene S. / ST131 fimH22 Escherichia coli isolate with a blaCMY-2/IncI1/ST12 plasmid obtained from a patient with bloodstream infection: highly similar to E. coli isolates of broiler origin. In: Journal of Antimicrobial Chemotherapy. 2019 ; Vol. 74, No. 3. pp. 557-560.
@article{2818b569f4a04769989fd2ef4a6edc1a,
title = "ST131 fimH22 Escherichia coli isolate with a blaCMY-2/IncI1/ST12 plasmid obtained from a patient with bloodstream infection: highly similar to E. coli isolates of broiler origin",
abstract = "Objectives: This study compares the genome of an ST131 CMY-2-producing Escherichia coli isolate from a Danish patient with other ST131 CMY-2-producing E. coli isolates of both human and animal origin.Methods: In 2016, an ST131 CMY-2-producing E. coli isolate (ESBL20160056) was obtained from a patient with a bloodstream infection. The genome of the ESBL20160056 isolate was compared with genomes from six ST131 CMY-2-producing E. coli isolates obtained from broiler meat imported to Denmark, 15 ST131 CMY-2-producing E. coli isolates obtained from Enterobase (http://enterobase.warwick.ac.uk) and two ST131 CMY-2-producing E. coli from European collaborators. The plasmid from ESBL20160056 was sequenced using a MinION Mk1B (Oxford Nanopore Technologies).Results: The E. coli isolate from the Danish patient clustered together with 13 other fimH22 ST131 CMY-2-producing E. coli isolates in a distinct clade. The clade consisted of genomes from six E. coli isolates from humans collected in Denmark, Spain, Cambodia and the USA, six E. coli isolates obtained from broiler meat samples imported to Denmark from France, the Netherlands and Germany, and two E. coli isolates obtained from broilers in Belgium and Luxembourg. The 101.5 kb plasmid with blaCMY-2 from ESBL20160056 had an IncI1 replicon and belonged to ST12 using the plasmid MLST scheme. In total, 10 of the 14 ST131 E. coli isolates belonging to the fimH22 clade carried an IncI1 ST12 plasmid with blaCMY-2.Conclusions: From our data, it seems plausible that the ST131 fimH22 CMY-2-producing E. coli isolate obtained from the Danish patient could have a zoonotic broiler origin.",
author = "Louise Roer and S{\o}ren Overballe-Petersen and Frank Hansen and Johannesen, {Thor B} and Marc Stegger and Valeria Bortolaia and Pimlapas Leekitcharoenphon and {Bisgaard Korsgaard}, Helle and Seyfarth, {Anne Mette} and Jo{\"e}l Mossong and Pierre Wattiau and C{\'e}cile Boland and Hansen, {Dennis S} and Henrik Hasman and Hammerum, {Anette M} and Hendriksen, {Rene S.}",
year = "2019",
doi = "10.1093/jac/dky484",
language = "English",
volume = "74",
pages = "557--560",
journal = "Journal of Antimicrobial Chemotherapy",
issn = "0305-7453",
publisher = "Oxford University Press",
number = "3",

}

ST131 fimH22 Escherichia coli isolate with a blaCMY-2/IncI1/ST12 plasmid obtained from a patient with bloodstream infection: highly similar to E. coli isolates of broiler origin. / Roer, Louise; Overballe-Petersen, Søren; Hansen, Frank; Johannesen, Thor B; Stegger, Marc; Bortolaia, Valeria; Leekitcharoenphon, Pimlapas; Bisgaard Korsgaard, Helle ; Seyfarth, Anne Mette; Mossong, Joël; Wattiau, Pierre; Boland, Cécile; Hansen, Dennis S; Hasman, Henrik; Hammerum, Anette M; Hendriksen, Rene S.

In: Journal of Antimicrobial Chemotherapy, Vol. 74, No. 3, 2019, p. 557-560.

Research output: Contribution to journalJournal articleResearchpeer-review

TY - JOUR

T1 - ST131 fimH22 Escherichia coli isolate with a blaCMY-2/IncI1/ST12 plasmid obtained from a patient with bloodstream infection: highly similar to E. coli isolates of broiler origin

AU - Roer, Louise

AU - Overballe-Petersen, Søren

AU - Hansen, Frank

AU - Johannesen, Thor B

AU - Stegger, Marc

AU - Bortolaia, Valeria

AU - Leekitcharoenphon, Pimlapas

AU - Bisgaard Korsgaard, Helle

AU - Seyfarth, Anne Mette

AU - Mossong, Joël

AU - Wattiau, Pierre

AU - Boland, Cécile

AU - Hansen, Dennis S

AU - Hasman, Henrik

AU - Hammerum, Anette M

AU - Hendriksen, Rene S.

PY - 2019

Y1 - 2019

N2 - Objectives: This study compares the genome of an ST131 CMY-2-producing Escherichia coli isolate from a Danish patient with other ST131 CMY-2-producing E. coli isolates of both human and animal origin.Methods: In 2016, an ST131 CMY-2-producing E. coli isolate (ESBL20160056) was obtained from a patient with a bloodstream infection. The genome of the ESBL20160056 isolate was compared with genomes from six ST131 CMY-2-producing E. coli isolates obtained from broiler meat imported to Denmark, 15 ST131 CMY-2-producing E. coli isolates obtained from Enterobase (http://enterobase.warwick.ac.uk) and two ST131 CMY-2-producing E. coli from European collaborators. The plasmid from ESBL20160056 was sequenced using a MinION Mk1B (Oxford Nanopore Technologies).Results: The E. coli isolate from the Danish patient clustered together with 13 other fimH22 ST131 CMY-2-producing E. coli isolates in a distinct clade. The clade consisted of genomes from six E. coli isolates from humans collected in Denmark, Spain, Cambodia and the USA, six E. coli isolates obtained from broiler meat samples imported to Denmark from France, the Netherlands and Germany, and two E. coli isolates obtained from broilers in Belgium and Luxembourg. The 101.5 kb plasmid with blaCMY-2 from ESBL20160056 had an IncI1 replicon and belonged to ST12 using the plasmid MLST scheme. In total, 10 of the 14 ST131 E. coli isolates belonging to the fimH22 clade carried an IncI1 ST12 plasmid with blaCMY-2.Conclusions: From our data, it seems plausible that the ST131 fimH22 CMY-2-producing E. coli isolate obtained from the Danish patient could have a zoonotic broiler origin.

AB - Objectives: This study compares the genome of an ST131 CMY-2-producing Escherichia coli isolate from a Danish patient with other ST131 CMY-2-producing E. coli isolates of both human and animal origin.Methods: In 2016, an ST131 CMY-2-producing E. coli isolate (ESBL20160056) was obtained from a patient with a bloodstream infection. The genome of the ESBL20160056 isolate was compared with genomes from six ST131 CMY-2-producing E. coli isolates obtained from broiler meat imported to Denmark, 15 ST131 CMY-2-producing E. coli isolates obtained from Enterobase (http://enterobase.warwick.ac.uk) and two ST131 CMY-2-producing E. coli from European collaborators. The plasmid from ESBL20160056 was sequenced using a MinION Mk1B (Oxford Nanopore Technologies).Results: The E. coli isolate from the Danish patient clustered together with 13 other fimH22 ST131 CMY-2-producing E. coli isolates in a distinct clade. The clade consisted of genomes from six E. coli isolates from humans collected in Denmark, Spain, Cambodia and the USA, six E. coli isolates obtained from broiler meat samples imported to Denmark from France, the Netherlands and Germany, and two E. coli isolates obtained from broilers in Belgium and Luxembourg. The 101.5 kb plasmid with blaCMY-2 from ESBL20160056 had an IncI1 replicon and belonged to ST12 using the plasmid MLST scheme. In total, 10 of the 14 ST131 E. coli isolates belonging to the fimH22 clade carried an IncI1 ST12 plasmid with blaCMY-2.Conclusions: From our data, it seems plausible that the ST131 fimH22 CMY-2-producing E. coli isolate obtained from the Danish patient could have a zoonotic broiler origin.

U2 - 10.1093/jac/dky484

DO - 10.1093/jac/dky484

M3 - Journal article

VL - 74

SP - 557

EP - 560

JO - Journal of Antimicrobial Chemotherapy

JF - Journal of Antimicrobial Chemotherapy

SN - 0305-7453

IS - 3

ER -