Spoilage fungi and their mycotoxins in commercially marketed chestnuts

David Patrick Overy, K.A. Seifert, M.E. Savard, Jens Christian Frisvad

    Research output: Contribution to journalJournal articleResearchpeer-review

    Abstract

    A nationwide survey was carried out to assess mould spoilage of Castanea sativa nuts sold in Canadian grocery stores in 1998-99. Morphological and cultural characters, along with secondary metabolite profiles derived from thin-layer chromatography, were used to sort and identify fungi cultured from nut tissue. Three mycotoxigenic fungi dominated (Penicillium crustosum, Penicillium glabrum/spinulosum and Penicillium discolor) and were isolated at frequencies of 67.1%, 18.6% and 17.7%, respectively, from a total sample size of 350 nuts. Another mycotoxin producer, Aspergillus ochraceus was also isolated, but at a much lower frequency. HPLC and diode array detection were used to confirm the suspected presence of the mycotoxins penitrem A, chactoglobosin A and C, emodin and ochratoxin A in extracts prepared from naturally infected nut tissue. To the best of our knowledge, this is the first time emodin has been found in a naturally contaminated food source.
    Original languageEnglish
    JournalInternational Journal of Food Microbiology
    Volume88
    Issue number1
    Pages (from-to)69-77
    ISSN0168-1605
    Publication statusPublished - 2003

    Fingerprint Dive into the research topics of 'Spoilage fungi and their mycotoxins in commercially marketed chestnuts'. Together they form a unique fingerprint.

    Cite this