@inbook{7c5055ec4df740fc8c5d08dde2a770ac,
title = "SpeedyGenesXL: an Automated, High-Throughput Platform for the Preparation of Bespoke Ultralarge Variant Libraries for Directed Evolution",
abstract = "Directed evolution of proteins is a highly effective strategy for tailoring biocatalysts to a particular application, and is capable of engineering improvements such as kcat, thermostability and organic solvent tolerance. It is recognized that large and systematic libraries are required to navigate a protein{\textquoteright}s vast and rugged sequence landscape effectively, yet their preparation is nontrivial and commercial libraries are extremely costly. To address this, we have developed SpeedyGenesXL, an automated, high-throughput platform for the production of wild-type genes, Boolean OR, combinatorial, or combinatorial-OR-type libraries based on the SpeedyGenes methodology. Together this offers a flexible platform for library synthesis, capable of generating many different bespoke, diverse libraries simultaneously.",
keywords = "Bespoke gene variant library synthesis, Automation, Directed evolution, Synthetic biology",
author = "Sadler, \{Joanna C.\} and Neil Swainston and Dunstan, \{Mark S.\} and Andrew Currin and Kell, \{Douglas B.\}",
year = "2022",
doi = "10.1007/978-1-0716-2152-3\_5",
language = "English",
volume = "2461",
series = "Methods in Molecular Biology",
publisher = "Humana Press",
pages = "67--83",
booktitle = "Directed Evolution",
}