Spatial patterns of Antimicrobial Resistance Genes in Danish Pig Farms

Anna Camilla Birkegård; A. K. Ersbøll; Tariq Hisham Beshara Halasa; Julie Clasen; Anders Folkesson; Håkan Vigre; Nils Toft

Spatial patterns of Antimicrobial Resistance Genes in Danish Pig Farms

Anna Camilla Birkegård, A. K. Ersbøll, Tariq Hisham Beshara Halasa, Julie Clasen, Anders Folkesson, Håkan Vigre, Nils Toft

University of Copenhagen

Research output: Chapter in Book/Report/Conference proceeding › Article in proceedings › Research › peer-review

Abstract

Samples from 687 Danish pig farms were collected at five finisher slaughterhouses in February and March 2015. Faecal samples from five pigs per farm were collected randomly at the slaughter line and pooled into one sample per farm. DNA was extracted from the pooled samples and the level of seven antimicrobial resistance genes, ermB, ermF, sulI, sulII, tet(M), tet(O) and tet(W), was quantified by a high-throughput qPCR. It was evaluated whether the sample method resulted in a study population representative of Danish pig farms with finishers where it was found that the study population was biased towards farms having more finisher and a higher productivity. Spatial cluster analyses were performed in SaTScan®. The results showed significant spatial clusters for ermF, ermB, sulII and tet(W) whereas no significant clusters were found for sulI, tet(M) and tet(O).

Original language	English
Title of host publication	Proceedings of the SVEPM: Annual Meeting 2016
Number of pages	10
Publication date	2016
Pages	182-191
ISBN (Electronic)	978-0-948073-35-9
Publication status	Published - 2016
Event	SVEPM: Annual Meeting 2016 - Konventum, Elsinore, Denmark Duration: 16 Mar 2016 → 18 Mar 2016

Conference

Conference	SVEPM
Location	Konventum
Country/Territory	Denmark
City	Elsinore
Period	16/03/2016 → 18/03/2016

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SPATIAL PATTERNS OF ANTIMICROBIAL RESISTANCE GENES IN DANISH PIG FARMS
Anna Camilla Birkegård (Speaker)
17 Mar 2016
Activity: Talks and presentations › Conference presentations

Cite this

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title = "Spatial patterns of Antimicrobial Resistance Genes in Danish Pig Farms",

abstract = "Samples from 687 Danish pig farms were collected at five finisher slaughterhouses in February and March 2015. Faecal samples from five pigs per farm were collected randomly at the slaughter line and pooled into one sample per farm. DNA was extracted from the pooled samples and the level of seven antimicrobial resistance genes, ermB, ermF, sulI, sulII, tet(M), tet(O) and tet(W), was quantified by a high-throughput qPCR. It was evaluated whether the sample method resulted in a study population representative of Danish pig farms with finishers where it was found that the study population was biased towards farms having more finisher and a higher productivity. Spatial cluster analyses were performed in SaTScan{\textregistered}. The results showed significant spatial clusters for ermF, ermB, sulII and tet(W) whereas no significant clusters were found for sulI, tet(M) and tet(O).",

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language = "English",

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T1 - Spatial patterns of Antimicrobial Resistance Genes in Danish Pig Farms

AU - Birkegård, Anna Camilla

AU - Ersbøll, A. K.

AU - Hisham Beshara Halasa, Tariq

AU - Clasen, Julie

AU - Folkesson, Anders

AU - Vigre, Håkan

AU - Toft, Nils

PY - 2016

Y1 - 2016

N2 - Samples from 687 Danish pig farms were collected at five finisher slaughterhouses in February and March 2015. Faecal samples from five pigs per farm were collected randomly at the slaughter line and pooled into one sample per farm. DNA was extracted from the pooled samples and the level of seven antimicrobial resistance genes, ermB, ermF, sulI, sulII, tet(M), tet(O) and tet(W), was quantified by a high-throughput qPCR. It was evaluated whether the sample method resulted in a study population representative of Danish pig farms with finishers where it was found that the study population was biased towards farms having more finisher and a higher productivity. Spatial cluster analyses were performed in SaTScan®. The results showed significant spatial clusters for ermF, ermB, sulII and tet(W) whereas no significant clusters were found for sulI, tet(M) and tet(O).

AB - Samples from 687 Danish pig farms were collected at five finisher slaughterhouses in February and March 2015. Faecal samples from five pigs per farm were collected randomly at the slaughter line and pooled into one sample per farm. DNA was extracted from the pooled samples and the level of seven antimicrobial resistance genes, ermB, ermF, sulI, sulII, tet(M), tet(O) and tet(W), was quantified by a high-throughput qPCR. It was evaluated whether the sample method resulted in a study population representative of Danish pig farms with finishers where it was found that the study population was biased towards farms having more finisher and a higher productivity. Spatial cluster analyses were performed in SaTScan®. The results showed significant spatial clusters for ermF, ermB, sulII and tet(W) whereas no significant clusters were found for sulI, tet(M) and tet(O).

M3 - Article in proceedings

SP - 182

EP - 191

BT - Proceedings of the SVEPM: Annual Meeting 2016

T2 - SVEPM

Y2 - 16 March 2016 through 18 March 2016

ER -

Spatial patterns of Antimicrobial Resistance Genes in Danish Pig Farms

Abstract

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SPATIAL PATTERNS OF ANTIMICROBIAL RESISTANCE GENES IN DANISH PIG FARMS

Cite this