Spatial patterns of Antimicrobial Resistance Genes in Danish Pig Farms

Anna Camilla Birkegård, A. K. Ersbøll, Tariq Hisham Beshara Halasa, Julie Clasen, Anders Folkesson, Håkan Vigre, Nils Toft

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Abstract

Samples from 687 Danish pig farms were collected at five finisher slaughterhouses in February and March 2015. Faecal samples from five pigs per farm were collected randomly at the slaughter line and pooled into one sample per farm. DNA was extracted from the pooled samples and the level of seven antimicrobial resistance genes, ermB, ermF, sulI, sulII, tet(M), tet(O) and tet(W), was quantified by a high-throughput qPCR. It was evaluated whether the sample method resulted in a study population representative of Danish pig farms with finishers where it was found that the study population was biased towards farms having more finisher and a higher productivity. Spatial cluster analyses were performed in SaTScan®. The results showed significant spatial clusters for ermF, ermB, sulII and tet(W) whereas no significant clusters were found for sulI, tet(M) and tet(O).
Original languageEnglish
Title of host publicationProceedings of the SVEPM: Annual Meeting 2016
Number of pages10
Publication date2016
Pages182-191
ISBN (Electronic)978-0-948073-35-9
Publication statusPublished - 2016
EventSVEPM: Annual Meeting 2016 - Konventum, Elsinore, Denmark
Duration: 16 Mar 201618 Mar 2016

Conference

ConferenceSVEPM
LocationKonventum
CountryDenmark
CityElsinore
Period16/03/201618/03/2016

Cite this

Birkegård, A. C., Ersbøll, A. K., Hisham Beshara Halasa, T., Clasen, J., Folkesson, A., Vigre, H., & Toft, N. (2016). Spatial patterns of Antimicrobial Resistance Genes in Danish Pig Farms. In Proceedings of the SVEPM: Annual Meeting 2016 (pp. 182-191)