Spatial Distribution of Escherichia coli in the Mouse Large Intestine Inferred from rRNA In Situ Hybridization

Lars Kærgaard Poulsen, Fusheng Lan, Claus Sternberg, Palle Hobolth, Søren Molin, Karen Angeliki Krogfelt

    Research output: Contribution to journalJournal articleResearchpeer-review

    Abstract

    Fluorescent oligonucleotide probes targeting rRNA were used to develop an in situ hybridization technique by which the spatial distribution of Escherichia coli in the large intestines of streptomycin-treated mice was determined. Single E. coli cells were identified in thin frozen sections from the large intestines by the use of a probe specific for E. coli 23S rRNA. Furthermore, the total bacterial population was visualized with an rRNA probe targeting the domain Bacteria. By this technique, all E. coli cells were seen embedded in the mucosal material overlying the epithelial cells of the large intestine, and no direct attachment to the epithelium was observed.
    Original languageEnglish
    JournalInfection and Immunity
    Volume62
    Issue number11
    Pages (from-to)5191-5194
    ISSN0019-9567
    Publication statusPublished - 1994

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