Sox9-dependent expression of Gstm6 in Sertoli cells during testis development in mice

Annemiek Beverdam, Terje Svingen, Stefan Bagheri-Fam, Pascal Bernard, Peter McClive, Mathew Robson, Mahdi Banan Khojasteh, Mahboubeh Salehi, Andrew H. Sinclair, Vincent R. Harley, Peter Koopman

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

Glutathione S-transferases (GSTs) are an important family of multifunctional enzymes that play a role in the protection of tissues by the detoxification of hazardous and carcinogenic compounds. We found previously that Gstm6 is upregulated in the somatic cells of male mouse fetal gonads relative to female gonads. In this study, we describe the spatial and temporal expression pattern of Gstm6 during mouse development. We show that Gstm6 is predominantly expressed in the reproductive system, at significantly higher levels in XY gonads compared with XX gonads from 11.5 dpc onwards, and remains expressed in the testes in adult mice. Its expression is associated with the Sertoli cell lineage, and is dependent on the expression of the male sex-determining gene Sox9. Our data suggest that Gstm6 plays a male-specific role in gonad development or function, possibly by modulating the exposure of somatic tissue and/or germ cells to endogenous or exogenous toxicants.
Original languageEnglish
JournalReproduction
Volume137
Issue number3
Pages (from-to)481-486
Number of pages6
ISSN1470-1626
DOIs
Publication statusPublished - 2009
Externally publishedYes

Keywords

  • Animals
  • Female
  • Gene Expression Profiling
  • Gene Expression Regulation, Developmental
  • Glutathione Transferase
  • In Situ Hybridization
  • Male
  • Mice
  • Mice, Inbred C57BL
  • Mice, Transgenic
  • Ovary
  • Reverse Transcriptase Polymerase Chain Reaction
  • SOX9 Transcription Factor
  • Sertoli Cells
  • Testis
  • Sox9 protein, mouse
  • EC 2.5.1.18 Glutathione Transferase
  • EC 2.5.1.18 glutathione S-transferase M1
  • EC 2.5.1.18 glutathione S-transferase Mu 2
  • glutathione transferase
  • glutathione transferase M6
  • transcription factor Sox9
  • unclassified drug
  • glutathione S-transferase M1
  • glutathione S-transferase Mu 2
  • glutathione transferase M1
  • glutathione transferase M2
  • animal cell
  • article
  • cell lineage
  • controlled study
  • embryo
  • mouse
  • nonhuman
  • priority journal
  • protein expression
  • protein function
  • quantitative analysis
  • reverse transcription polymerase chain reaction
  • Sertoli cell
  • sex determination
  • testis development
  • upregulation
  • animal
  • C57BL mouse
  • female
  • gene expression profiling
  • gene expression regulation
  • genetics
  • in situ hybridization
  • male
  • metabolism
  • methodology
  • ovary
  • prenatal development
  • testis
  • transgenic mouse
  • Mus
  • DEVELOPMENTAL
  • REPRODUCTIVE
  • SEX DETERMINATION REVEALS
  • MOUSE TESTIS
  • GENE-EXPRESSION
  • CANDIDATE GENES
  • DIFFERENTIATION
  • SOX9
  • SRY
  • PROLIFERATION
  • GRANULOSA
  • PROMOTER
  • spatial pattern
  • temporal pattern
  • Rodentia Mammalia Vertebrata Chordata Animalia (Animals, Chordates, Mammals, Nonhuman Vertebrates, Nonhuman Mammals, Rodents, Vertebrates) - Muridae [86375] mouse common adult female, male
  • mouse Gstm6 gene [Muridae] mouse glutathione S-transferase, mu 6 gene expression
  • mouse sox9 gene [Muridae] mouse SRY gene expression
  • glutathione S-transferase 50812-37-8
  • 02506, Cytology - Animal
  • 03502, Genetics - General
  • 03506, Genetics - Animal
  • 10802, Enzymes - General and comparative studies: coenzymes
  • 16504, Reproductive system - Physiology and biochemistry
  • 17002, Endocrine - General
  • 17006, Endocrine - Gonads and placenta
  • 25502, Development and Embryology - General and descriptive
  • Biochemistry and Molecular Biophysics
  • Reproduction
  • germ cell reproductive system
  • gonad reproductive system, endocrine system
  • Sertoli cell reproductive system
  • testis reproductive system
  • Development
  • Molecular Genetics
  • Reproductive System

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