@inbook{99175b483fb74897bc3b709e3f34591e,
title = "Solid-Phase Hybridization Assay for Detection of Mutated Cancer DNA by Fluorescence",
abstract = "We report a straightforward protocol for the detection of mutated DNA extracted from cancer cells. The assay combines a step-wise solid-phase hybridization and a readout by fluorescence emission. We detect a single-nucleotide polymorphism in two human oncogenes, BRAF and EGFR, and reach a limit of the detection of 300 pM by conventional fluorometry. The protocol described herein may be used as a foundation for development of automatic optimized assays capable for detection of mutant DNA and RNA in vitro and in cells.",
keywords = "DNA oncogene, Fluorescent oligonucleotides, Hybridization assay, Fluorometry",
author = "Maria Taskova and Kira Astakhova",
year = "2020",
doi = "10.1007/978-1-0716-0138-9_4",
language = "English",
isbn = "978-1-0716-0137-2",
volume = "2063",
series = "Methods in Molecular Biology",
publisher = "Springer",
pages = "37--44",
editor = "{Kira Astakhova} and {Syeda Atia Bukhari}",
booktitle = "Nucleic Acid Detection and Structural Investigations",
}
