Small-angle scattering studies on clathrin-coated vesicles

R. Bauer, M. Behan, S. Hansen, G. Jones, K. Mortensen, T. Særmark, L. Øgendal

    Research output: Contribution to journalJournal articleResearchpeer-review

    Abstract

    Structural information of clathrin-coated vesicles has been achieved by small-angle X-ray, neutron and dynamic light scattering studies. A characteristic peak in the X-ray and neutron scattering profile (in D2O) from intact coated vesiles is consistent with the polygonic structure of the clathrin coat. Neutron as well as dynamic light scattering gives a coated vesicle size close to 1000 A. Dynamic light scattering detects a distribution of sizes for the coated vesicles demonstrating polydispersity of the samples. Quick freezing and slow thawing cause breakdown of the polygonic coat and production of large aggregates, as observed by dynamic light scattering and the reduction of the peak in the X-ray scattering profile as well as an increase in the scattering intensity at the lowest angles in the neutron scattering profile.
    Original languageEnglish
    JournalJournal of Applied Crystallography
    Volume24
    Issue number5
    Pages (from-to)815-821
    ISSN0021-8898
    DOIs
    Publication statusPublished - 1991

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