SMAC mimetics promote NIK-dependent inhibition of CD4+ TH17 cell differentiation

John Rizk, Joseph Kaplinsky, Rasmus Agerholm, Darshana Dattatraya Kadekar, Fredrik Ivars, William W. Agace, W. Wei-Lynn Wong, Matthew J. Szucs, Samuel A. Myers, Steven A. Carr, Ari Waisman, Vasileios Bekiaris*

*Corresponding author for this work

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

Second mitochondria-derived activator of caspase (SMAC) mimetics (SMs) are selective antagonists of the inhibitor of apoptosis proteins (IAPs), which activate noncanonical NF-κB signaling and promote tumor cell death. Through gene expression analysis, we found that treatment of CD4+ T cells with SMs during T helper 17 (TH17) cell differentiation disrupted the balance between two antagonistic transcription factor modules. Moreover, proteomics analysis revealed that SMs altered the abundance of proteins associated with cell cycle, mitochondrial activity, and the balance between canonical and noncanonical NF-κB signaling. Whereas SMs inhibited interleukin-17 (IL-17) production and ameliorated TH17 cell-driven inflammation, they stimulated IL-22 secretion. Mechanistically, SM-mediated activation of NF-κB-inducing kinase (NIK) and the transcription factors RelB and p52 directly suppressed Il17a expression and IL-17A protein production, as well as the expression of a number of other immune genes. Induction of IL-22 production correlated with the NIK-dependent reduction in cMAF protein abundance and the enhanced activity of the aryl hydrocarbon receptor. Last, SMs also increased IL-9 and IL-13 production and, under competing conditions, favored the differentiation of naïve CD4+ T cells into TH2 cells rather than TH17 cells. These results demonstrate that SMs shape the gene expression and protein profiles of TH17 cells and inhibit TH17 cell-driven autoimmunity.
Original languageEnglish
Article numbereaaw3469
JournalScience Signaling
Volume12
Issue number596
Number of pages15
ISSN1945-0877
DOIs
Publication statusPublished - 2019

Cite this

Rizk, John ; Kaplinsky, Joseph ; Agerholm, Rasmus ; Kadekar, Darshana Dattatraya ; Ivars, Fredrik ; Agace, William W. ; Wong, W. Wei-Lynn ; Szucs, Matthew J. ; Myers, Samuel A. ; Carr, Steven A. ; Waisman, Ari ; Bekiaris, Vasileios. / SMAC mimetics promote NIK-dependent inhibition of CD4+ TH17 cell differentiation. In: Science Signaling. 2019 ; Vol. 12, No. 596.
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title = "SMAC mimetics promote NIK-dependent inhibition of CD4+ TH17 cell differentiation",
abstract = "Second mitochondria-derived activator of caspase (SMAC) mimetics (SMs) are selective antagonists of the inhibitor of apoptosis proteins (IAPs), which activate noncanonical NF-κB signaling and promote tumor cell death. Through gene expression analysis, we found that treatment of CD4+ T cells with SMs during T helper 17 (TH17) cell differentiation disrupted the balance between two antagonistic transcription factor modules. Moreover, proteomics analysis revealed that SMs altered the abundance of proteins associated with cell cycle, mitochondrial activity, and the balance between canonical and noncanonical NF-κB signaling. Whereas SMs inhibited interleukin-17 (IL-17) production and ameliorated TH17 cell-driven inflammation, they stimulated IL-22 secretion. Mechanistically, SM-mediated activation of NF-κB-inducing kinase (NIK) and the transcription factors RelB and p52 directly suppressed Il17a expression and IL-17A protein production, as well as the expression of a number of other immune genes. Induction of IL-22 production correlated with the NIK-dependent reduction in cMAF protein abundance and the enhanced activity of the aryl hydrocarbon receptor. Last, SMs also increased IL-9 and IL-13 production and, under competing conditions, favored the differentiation of na{\"i}ve CD4+ T cells into TH2 cells rather than TH17 cells. These results demonstrate that SMs shape the gene expression and protein profiles of TH17 cells and inhibit TH17 cell-driven autoimmunity.",
author = "John Rizk and Joseph Kaplinsky and Rasmus Agerholm and Kadekar, {Darshana Dattatraya} and Fredrik Ivars and Agace, {William W.} and Wong, {W. Wei-Lynn} and Szucs, {Matthew J.} and Myers, {Samuel A.} and Carr, {Steven A.} and Ari Waisman and Vasileios Bekiaris",
year = "2019",
doi = "10.1126/scisignal.aaw3469",
language = "English",
volume = "12",
journal = "Science Signaling",
issn = "1945-0877",
publisher = "American Association for the Advancement of Science",
number = "596",

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Rizk, J, Kaplinsky, J, Agerholm, R, Kadekar, DD, Ivars, F, Agace, WW, Wong, WW-L, Szucs, MJ, Myers, SA, Carr, SA, Waisman, A & Bekiaris, V 2019, 'SMAC mimetics promote NIK-dependent inhibition of CD4+ TH17 cell differentiation', Science Signaling, vol. 12, no. 596, eaaw3469. https://doi.org/10.1126/scisignal.aaw3469

SMAC mimetics promote NIK-dependent inhibition of CD4+ TH17 cell differentiation. / Rizk, John; Kaplinsky, Joseph; Agerholm, Rasmus; Kadekar, Darshana Dattatraya; Ivars, Fredrik; Agace, William W.; Wong, W. Wei-Lynn; Szucs, Matthew J.; Myers, Samuel A.; Carr, Steven A.; Waisman, Ari; Bekiaris, Vasileios.

In: Science Signaling, Vol. 12, No. 596, eaaw3469, 2019.

Research output: Contribution to journalJournal articleResearchpeer-review

TY - JOUR

T1 - SMAC mimetics promote NIK-dependent inhibition of CD4+ TH17 cell differentiation

AU - Rizk, John

AU - Kaplinsky, Joseph

AU - Agerholm, Rasmus

AU - Kadekar, Darshana Dattatraya

AU - Ivars, Fredrik

AU - Agace, William W.

AU - Wong, W. Wei-Lynn

AU - Szucs, Matthew J.

AU - Myers, Samuel A.

AU - Carr, Steven A.

AU - Waisman, Ari

AU - Bekiaris, Vasileios

PY - 2019

Y1 - 2019

N2 - Second mitochondria-derived activator of caspase (SMAC) mimetics (SMs) are selective antagonists of the inhibitor of apoptosis proteins (IAPs), which activate noncanonical NF-κB signaling and promote tumor cell death. Through gene expression analysis, we found that treatment of CD4+ T cells with SMs during T helper 17 (TH17) cell differentiation disrupted the balance between two antagonistic transcription factor modules. Moreover, proteomics analysis revealed that SMs altered the abundance of proteins associated with cell cycle, mitochondrial activity, and the balance between canonical and noncanonical NF-κB signaling. Whereas SMs inhibited interleukin-17 (IL-17) production and ameliorated TH17 cell-driven inflammation, they stimulated IL-22 secretion. Mechanistically, SM-mediated activation of NF-κB-inducing kinase (NIK) and the transcription factors RelB and p52 directly suppressed Il17a expression and IL-17A protein production, as well as the expression of a number of other immune genes. Induction of IL-22 production correlated with the NIK-dependent reduction in cMAF protein abundance and the enhanced activity of the aryl hydrocarbon receptor. Last, SMs also increased IL-9 and IL-13 production and, under competing conditions, favored the differentiation of naïve CD4+ T cells into TH2 cells rather than TH17 cells. These results demonstrate that SMs shape the gene expression and protein profiles of TH17 cells and inhibit TH17 cell-driven autoimmunity.

AB - Second mitochondria-derived activator of caspase (SMAC) mimetics (SMs) are selective antagonists of the inhibitor of apoptosis proteins (IAPs), which activate noncanonical NF-κB signaling and promote tumor cell death. Through gene expression analysis, we found that treatment of CD4+ T cells with SMs during T helper 17 (TH17) cell differentiation disrupted the balance between two antagonistic transcription factor modules. Moreover, proteomics analysis revealed that SMs altered the abundance of proteins associated with cell cycle, mitochondrial activity, and the balance between canonical and noncanonical NF-κB signaling. Whereas SMs inhibited interleukin-17 (IL-17) production and ameliorated TH17 cell-driven inflammation, they stimulated IL-22 secretion. Mechanistically, SM-mediated activation of NF-κB-inducing kinase (NIK) and the transcription factors RelB and p52 directly suppressed Il17a expression and IL-17A protein production, as well as the expression of a number of other immune genes. Induction of IL-22 production correlated with the NIK-dependent reduction in cMAF protein abundance and the enhanced activity of the aryl hydrocarbon receptor. Last, SMs also increased IL-9 and IL-13 production and, under competing conditions, favored the differentiation of naïve CD4+ T cells into TH2 cells rather than TH17 cells. These results demonstrate that SMs shape the gene expression and protein profiles of TH17 cells and inhibit TH17 cell-driven autoimmunity.

U2 - 10.1126/scisignal.aaw3469

DO - 10.1126/scisignal.aaw3469

M3 - Journal article

C2 - 31455723

VL - 12

JO - Science Signaling

JF - Science Signaling

SN - 1945-0877

IS - 596

M1 - eaaw3469

ER -