Single-Molecule Encapsulation: A Straightforward Route to Highly Stable and Printable Enzymes

Ana Beloqui; Sarah Baur; Vanessa Trouillet; Alexander Welle; Peter Jeppe Madsen; Martin Bastmeyer; Guillaume Delaittre

doi:10.1002/smll.201503405

Single-Molecule Encapsulation: A Straightforward Route to Highly Stable and Printable Enzymes

Ana Beloqui, Sarah Baur, Vanessa Trouillet, Alexander Welle, Peter Jeppe Madsen, Martin Bastmeyer, Guillaume Delaittre

Research output: Contribution to journal › Journal article › Research › peer-review

Abstract

A mild, fast, and sequence-independent method for controlled enzyme immobilization is presented. This novel approach involves the encapsulation of single-enzyme molecules and the covalent attachment of these nanobiocatalysts onto surfaces. Fast and mild immobilization conditions, combined with low nonspecific adsorption on hydrophobic substrates, enables well-defined surface patterns via microcontact printing. The biohybrid materials show enhanced activity in organic solvents.

Original language	English
Journal	Small
Volume	12
Issue number	13
Pages (from-to)	1716-1722
ISSN	1613-6810
DOIs	https://doi.org/10.1002/smll.201503405
Publication status	Published - 2016
Externally published	Yes

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title = "Single-Molecule Encapsulation: A Straightforward Route to Highly Stable and Printable Enzymes",

abstract = "A mild, fast, and sequence-independent method for controlled enzyme immobilization is presented. This novel approach involves the encapsulation of single-enzyme molecules and the covalent attachment of these nanobiocatalysts onto surfaces. Fast and mild immobilization conditions, combined with low nonspecific adsorption on hydrophobic substrates, enables well-defined surface patterns via microcontact printing. The biohybrid materials show enhanced activity in organic solvents.",

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AU - Baur, Sarah

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AU - Welle, Alexander

AU - Jeppe Madsen, Peter

AU - Bastmeyer, Martin

AU - Delaittre, Guillaume

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AB - A mild, fast, and sequence-independent method for controlled enzyme immobilization is presented. This novel approach involves the encapsulation of single-enzyme molecules and the covalent attachment of these nanobiocatalysts onto surfaces. Fast and mild immobilization conditions, combined with low nonspecific adsorption on hydrophobic substrates, enables well-defined surface patterns via microcontact printing. The biohybrid materials show enhanced activity in organic solvents.

U2 - 10.1002/smll.201503405

DO - 10.1002/smll.201503405

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Single-Molecule Encapsulation: A Straightforward Route to Highly Stable and Printable Enzymes

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