Single-cell technologies to understand the mechanisms of cellular adaption in chemostats

Naia Risager Wright, Nanna P. Rønnest, Nikolaus Sonnenschein*

*Corresponding author for this work

Research output: Contribution to journalJournal articleResearchpeer-review

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Abstract

There is a growing interest in continuous manufacturing within the bioprocessing community. In this context, the chemostat process is an important unit operation. The current application of chemostat processes in industry is limited although many high yielding processes are reported in literature. In order to reach the full potential of the chemostat in continuous manufacture, the output should be constant. However, adaptation is often observed resulting in changed productivities over time. The observed adaptation can be coupled to the selective pressure of the nutrient-limited environment in the chemostat. We argue that population heterogeneity should be taken into account when studying adaptation in the chemostat. We propose to investigate adaptation at the single-cell level and discuss the potential of different single-cell technologies, which could be used to increase the understanding of the phenomena. Currently, none of the discussed single-cell technologies fulfill all our criteria but in combination they may reveal important information, which can be used to understand and potentially control the adaptation.
Original languageEnglish
Article number579841
JournalFrontiers in Bioengineering and Biotechnology
Volume8
Number of pages10
ISSN2296-4185
DOIs
Publication statusPublished - 2020

Keywords

  • Chemostat cultivation
  • Continuous biomanufacturing
  • Adaption
  • Population heterogeneity
  • Microbes
  • Single-cell technologies

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