Abstract
Introduction. Serum 25-hydroxy-vitamin D is the established biomarker of vitamin D status although serum concentrations of vitamin D and 24,25-dihydroxyvitamin D may also be of interest to understand the in vivo kinetics of serum 25-hydroxyvitamin D. Method. An LC-MS/MS method was developed and validated to quantify vitamin D-3, 25-hydroxyvitamin D-3 and 24,25-dihydroxyvitamin D-3 in serum. After protein precipitation of the serum it was loaded on a HybridSPE column to separate vitamin D metabolites from phospholipids. Vitamin D-3, 25-hydroxyvitamin D-3 and 24,25-dihydroxyvitamin D-3 in the eluate were derivatized by 4-phenyl-1,2,4-triazoline-3,5-dione to improve sensitivity in the following LC-MS/MS analysis. Results. Using only 100 L serum the limit of quantification was
Original language | English |
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Journal | Scandinavian Journal of Clinical & Laboratory Investigation |
Volume | 74 |
Issue number | 5 |
Pages (from-to) | 418-423 |
Number of pages | 6 |
ISSN | 0036-5513 |
DOIs | |
Publication status | Published - 2014 |
Keywords
- MEDICINE,
- TANDEM MASS-SPECTROMETRY
- LIQUID-CHROMATOGRAPHY
- D METABOLITES
- CIRCULATING VITAMIN-D-3
- SUPPLEMENTATION
- Liquid chromatography
- vitamin D
- mass spectroscopy