Simultaneous quantification of multiple bacterial metabolites using surface-enhanced Raman scattering

Lidia Morelli*, Francesca Alessandra Centorbi, Oleksii Ilchenko, Christian Bille Jendresen, Danilo Demarchi, Alex Toftgaard Nielsen, Kinga Zor, Anja Boisen

*Corresponding author for this work

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

Given the commercial importance of the compounds produced by genetically modified organisms, there is a need for screening methods which facilitate the evaluation of newly developed strains, especially during the phase of proof-of-concept development. We report a time-efficient analysis method for the screening of bacterial strains, which enables the detection of two structurally similar secondary bacterial metabolites. By combining liquid-liquid extraction and surface-enhanced Raman scattering we were able to quantify p-coumaric acid and cinnamic acid, produced by genetically modified E. coli from tyrosine and phenylalanine, respectively. With the simple sample pre-treatment method, and by applying a partial least squares data analysis method, we simultaneously detected the analytes from four E. coli strains cultured in the presence or absence of tyrosine and phenylalanine.
Original languageEnglish
JournalAnalyst
Volume144
Issue number5
Pages (from-to)1600-1607
Number of pages8
ISSN0003-2654
DOIs
Publication statusPublished - 2019

Cite this

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title = "Simultaneous quantification of multiple bacterial metabolites using surface-enhanced Raman scattering",
abstract = "Given the commercial importance of the compounds produced by genetically modified organisms, there is a need for screening methods which facilitate the evaluation of newly developed strains, especially during the phase of proof-of-concept development. We report a time-efficient analysis method for the screening of bacterial strains, which enables the detection of two structurally similar secondary bacterial metabolites. By combining liquid-liquid extraction and surface-enhanced Raman scattering we were able to quantify p-coumaric acid and cinnamic acid, produced by genetically modified E. coli from tyrosine and phenylalanine, respectively. With the simple sample pre-treatment method, and by applying a partial least squares data analysis method, we simultaneously detected the analytes from four E. coli strains cultured in the presence or absence of tyrosine and phenylalanine.",
author = "Lidia Morelli and Centorbi, {Francesca Alessandra} and Oleksii Ilchenko and Jendresen, {Christian Bille} and Danilo Demarchi and Nielsen, {Alex Toftgaard} and Kinga Zor and Anja Boisen",
year = "2019",
doi = "10.1039/C8AN02128G",
language = "English",
volume = "144",
pages = "1600--1607",
journal = "Analyst",
issn = "0003-2654",
publisher = "Royal Society of Chemistry",
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Simultaneous quantification of multiple bacterial metabolites using surface-enhanced Raman scattering. / Morelli, Lidia; Centorbi, Francesca Alessandra ; Ilchenko, Oleksii; Jendresen, Christian Bille; Demarchi, Danilo; Nielsen, Alex Toftgaard; Zor, Kinga; Boisen, Anja.

In: Analyst, Vol. 144, No. 5, 2019, p. 1600-1607.

Research output: Contribution to journalJournal articleResearchpeer-review

TY - JOUR

T1 - Simultaneous quantification of multiple bacterial metabolites using surface-enhanced Raman scattering

AU - Morelli, Lidia

AU - Centorbi, Francesca Alessandra

AU - Ilchenko, Oleksii

AU - Jendresen, Christian Bille

AU - Demarchi, Danilo

AU - Nielsen, Alex Toftgaard

AU - Zor, Kinga

AU - Boisen, Anja

PY - 2019

Y1 - 2019

N2 - Given the commercial importance of the compounds produced by genetically modified organisms, there is a need for screening methods which facilitate the evaluation of newly developed strains, especially during the phase of proof-of-concept development. We report a time-efficient analysis method for the screening of bacterial strains, which enables the detection of two structurally similar secondary bacterial metabolites. By combining liquid-liquid extraction and surface-enhanced Raman scattering we were able to quantify p-coumaric acid and cinnamic acid, produced by genetically modified E. coli from tyrosine and phenylalanine, respectively. With the simple sample pre-treatment method, and by applying a partial least squares data analysis method, we simultaneously detected the analytes from four E. coli strains cultured in the presence or absence of tyrosine and phenylalanine.

AB - Given the commercial importance of the compounds produced by genetically modified organisms, there is a need for screening methods which facilitate the evaluation of newly developed strains, especially during the phase of proof-of-concept development. We report a time-efficient analysis method for the screening of bacterial strains, which enables the detection of two structurally similar secondary bacterial metabolites. By combining liquid-liquid extraction and surface-enhanced Raman scattering we were able to quantify p-coumaric acid and cinnamic acid, produced by genetically modified E. coli from tyrosine and phenylalanine, respectively. With the simple sample pre-treatment method, and by applying a partial least squares data analysis method, we simultaneously detected the analytes from four E. coli strains cultured in the presence or absence of tyrosine and phenylalanine.

U2 - 10.1039/C8AN02128G

DO - 10.1039/C8AN02128G

M3 - Journal article

VL - 144

SP - 1600

EP - 1607

JO - Analyst

JF - Analyst

SN - 0003-2654

IS - 5

ER -