Simultaneous quantification of multiple bacterial metabolites using surface-enhanced Raman scattering

Lidia Morelli*, Francesca Alessandra Centorbi, Oleksii Ilchenko, Christian Bille Jendresen, Danilo Demarchi, Alex Toftgaard Nielsen, Kinga Zor, Anja Boisen

*Corresponding author for this work

Research output: Contribution to journalJournal articleResearchpeer-review


Given the commercial importance of the compounds produced by genetically modified organisms, there is a need for screening methods which facilitate the evaluation of newly developed strains, especially during the phase of proof-of-concept development. We report a time-efficient analysis method for the screening of bacterial strains, which enables the detection of two structurally similar secondary bacterial metabolites. By combining liquid-liquid extraction and surface-enhanced Raman scattering we were able to quantify p-coumaric acid and cinnamic acid, produced by genetically modified E. coli from tyrosine and phenylalanine, respectively. With the simple sample pre-treatment method, and by applying a partial least squares data analysis method, we simultaneously detected the analytes from four E. coli strains cultured in the presence or absence of tyrosine and phenylalanine.
Original languageEnglish
Issue number5
Pages (from-to)1600-1607
Number of pages8
Publication statusPublished - 2019

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