Simultaneous measurement of two enzyme activities using infrared spectroscopy: A comparative evaluation of PARAFAC, TUCKER and N-PLS modeling

Andreas Baum, Per Waaben Hansen, Anne S. Meyer, Jørn Dalgaard Mikkelsen

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

Enzymes are used in many processes to release fermentable sugars for green production of biofuel, or the refinery of biomass for extraction of functional food ingredients such as pectin or prebiotic oligosaccharides. The complex biomasses may, however, require a multitude of specific enzymes which are active on specific substrates generating a multitude of products. In this paper we use the plant polymer, pectin, to present a method to quantify enzyme activity of two pectolytic enzymes by monitoring their superimposed spectral evolutions simultaneously. The data is analyzed by three chemometric multiway methods, namely PARAFAC, TUCKER3 and N-PLS, to establish simultaneous enzyme activity assays for pectin lyase and pectin methyl esterase. Correlation coefficients Rpred2 for prediction test sets are 0.48, 0.96 and 0.96 for pectin lyase and 0.70, 0.89 and 0.89 for pectin methyl esterase, respectively. The retrieved models are compared and prediction test sets show that especially TUCKER3 performs well, even in comparison to the supervised regression method N-PLS.
Original languageEnglish
JournalAnalytica Chimica Acta
Volume790
Pages (from-to)14-23
ISSN0003-2670
DOIs
Publication statusPublished - 2013

Keywords

  • Fourier transform infrared spectroscopy
  • Enzyme activity
  • Spectral evolution profiles
  • Multiway
  • Second-order calibration

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