Simultaneous determination of ochratoxin A, mycophenolic acid and fumonisin B-2 in meat products

Louise Marie Sørensen, Jesper Mogensen, Kristian Fog Nielsen

    Research output: Contribution to journalJournal articleResearchpeer-review

    Abstract

    Here we present a method for simultaneous determination of the fungal metabolites mycophenolic acid, ochratoxin A (OTA) and fumonisin B-2 (FB2) in meat products. Extraction was performed with water-acetonitrile, followed by acetone-induced precipitation of salts and proteins. Purification and identification of analytes was performed by mixed-mode reversed-phase anion-exchange chromatography in direct ion-exchange mode, followed by liquid chromatography-tandem mass spectrometry (LC-MS/MS) detection. Quantification was based on isotope dilution with fully C-13-labelled FB2 and OTA, and matrix-spiked calibration curves. Fermented sausages inoculated with an OTA- and FB2-producing strain of Aspergillus niger were analysed, but no analytes were detected. Analysis of 22 retail products showed one Parma meat with a very high level of OTA contamination (56-158 mu g/kg) that clearly exceeded the Italian regulatory limit of 1 mu g/kg. This sample and uninfected control samples were subsequently reanalysed, and the high OTA content was verified by two other techniques: (i) LC-time-of-flight MS confirmed the accurate mass as well as chlorine isotope pattern; and (ii) sample methylation in methanol-BF3 and subsequent LC-MS/MS provided indirect confirmation by detection of the OTA methyl ester. In the contaminated Parma ham, the high OTA level most likely originated from growth of Penicillium nordicum on the meat.
    Original languageEnglish
    JournalAnalytical and Bioanalytical Chemistry
    Volume398
    Issue number3
    Pages (from-to)1535-1542
    ISSN1618-2642
    DOIs
    Publication statusPublished - 2010

    Keywords

    • Meat products
    • Filamentous fungi
    • Mixed-mode reversed-phase anion exchange
    • Mycotoxins
    • LC-MS/MS
    • Isotope dilution

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