Simultaneous determination of gene expression and bacterial identity in single cells in defined mixtures of pure cultures

Lars K. Poulsen, Helen M. Dalton, Mark Angels, Kevin C. Marshall, Søren Molin, Amanda E. Goodman

    Research output: Contribution to journalJournal articleResearchpeer-review

    Abstract

    A protocol was developed to achieve the simultaneous determination of gene expression and bacterial identity at the level of single cells: a chromogenic beta-galactosidase activity assay was combined with in situ hybridization of Fluorescently labelled oligonucleotide probes to rRNA. The method allows monitoring of gene expression and quantification of beta-galactosidase activity in single cells.
    Original languageEnglish
    JournalApplied and Environmental Microbiology
    Volume63
    Issue number9
    Pages (from-to)3698-3702
    ISSN0099-2240
    Publication statusPublished - 1997

    Fingerprint Dive into the research topics of 'Simultaneous determination of gene expression and bacterial identity in single cells in defined mixtures of pure cultures'. Together they form a unique fingerprint.

    Cite this