Simultaneous determination of gene expression and bacterial identity in single cells in defined mixtures of pure cultures

Lars K. Poulsen; Helen M. Dalton; Mark Angels; Kevin C. Marshall; Søren Molin; Amanda E. Goodman

Simultaneous determination of gene expression and bacterial identity in single cells in defined mixtures of pure cultures

Lars K. Poulsen, Helen M. Dalton, Mark Angels, Kevin C. Marshall, Søren Molin, Amanda E. Goodman

Research output: Contribution to journal › Journal article › Research › peer-review

Abstract

A protocol was developed to achieve the simultaneous determination of gene expression and bacterial identity at the level of single cells: a chromogenic beta-galactosidase activity assay was combined with in situ hybridization of Fluorescently labelled oligonucleotide probes to rRNA. The method allows monitoring of gene expression and quantification of beta-galactosidase activity in single cells.

Original language	English
Journal	Applied and Environmental Microbiology
Volume	63
Issue number	9
Pages (from-to)	3698-3702
ISSN	0099-2240
Publication status	Published - 1997

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title = "Simultaneous determination of gene expression and bacterial identity in single cells in defined mixtures of pure cultures",

abstract = "A protocol was developed to achieve the simultaneous determination of gene expression and bacterial identity at the level of single cells: a chromogenic beta-galactosidase activity assay was combined with in situ hybridization of Fluorescently labelled oligonucleotide probes to rRNA. The method allows monitoring of gene expression and quantification of beta-galactosidase activity in single cells. ",

author = "Poulsen, {Lars K.} and Dalton, {Helen M.} and Mark Angels and Marshall, {Kevin C.} and S{\o}ren Molin and Goodman, {Amanda E.}",

year = "1997",

language = "English",

volume = "63",

pages = "3698--3702",

journal = "Applied and Environmental Microbiology",

issn = "0099-2240",

publisher = "American Society for Microbiology",

number = "9",

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TY - JOUR

T1 - Simultaneous determination of gene expression and bacterial identity in single cells in defined mixtures of pure cultures

AU - Poulsen, Lars K.

AU - Dalton, Helen M.

AU - Angels, Mark

AU - Marshall, Kevin C.

AU - Molin, Søren

AU - Goodman, Amanda E.

PY - 1997

Y1 - 1997

N2 - A protocol was developed to achieve the simultaneous determination of gene expression and bacterial identity at the level of single cells: a chromogenic beta-galactosidase activity assay was combined with in situ hybridization of Fluorescently labelled oligonucleotide probes to rRNA. The method allows monitoring of gene expression and quantification of beta-galactosidase activity in single cells.

AB - A protocol was developed to achieve the simultaneous determination of gene expression and bacterial identity at the level of single cells: a chromogenic beta-galactosidase activity assay was combined with in situ hybridization of Fluorescently labelled oligonucleotide probes to rRNA. The method allows monitoring of gene expression and quantification of beta-galactosidase activity in single cells.

M3 - Journal article

SN - 0099-2240

VL - 63

SP - 3698

EP - 3702

JO - Applied and Environmental Microbiology

JF - Applied and Environmental Microbiology

IS - 9

ER -

Simultaneous determination of gene expression and bacterial identity in single cells in defined mixtures of pure cultures

Abstract

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