Simulation and experimental validation of a SU-8 based PCR thermocycler chip with integrated heaters and temperature sensor

Jamil El-Ali, Ivan R. Perch-Nielsen, Claus Riber Poulsen, Dang Duong Bang, Pieter Telleman, Anders Wolff

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    Abstract

    We present a SU-8 based polymerase chain reaction (PCR) chip with integrated platinum thin film heaters and temperature sensor. The device is fabricated in SU-8 on a glass substrate. The use of SU-8 provides a simple microfabrication process for the PCR chamber, controllable surface properties and can allow on chip integration to other SU-8 based functional elements. Finite element modeling (FEM) and experiments show that the temperature distribution in the PCR chamber is homogeneous and that the chip is capable of fast thermal cycling. With heating and cooling rates of up to 50 and 30 degreesC/s, respectively, the performance of the chip is comparable with the best silicon micromachined PCR chips presented in the literature. The SU-8 chamber surface was found to be PCR compatible by amplification of yeast gene ribosomal protein S3 and Campylobacter gene cadF. The PCR compatibility of the chamber surfaces was enhanced by silanization.
    Original languageEnglish
    JournalSensors and Actuators A: Physical
    Volume110
    Issue number1-3
    Pages (from-to)3-10
    ISSN0924-4247
    DOIs
    Publication statusPublished - 2004

    Keywords

    • thermocycler
    • PCR chip
    • SU-8
    • FEM simulation

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