Simple fibre based dispersion management for two-photon excited fluorescence imaging through an endoscope

Konstantinos Dimopoulos, Dominik Marti, Peter E. Andersen

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Abstract

We want to implement two-photon excitation fluorescence microscopy (TPEFM) into endoscopes, since TPEFM can provide relevant biomarkers for cancer staging and grading in hollow organs, endoscopically accessible through natural orifices. However, many obstacles must be overcome, among others the delivery of short laser
pulses to the distal end of the endoscope. To this avail, we present imaging results using an all-fibre dispersion management scheme in a TPEFM setup. The scheme has been conceived by Jespersen et al. in 20101 and relies on the combination of a single mode fibre with normal and a higher order mode fibre with anomalous dispersion properties, fused in series using a long period grating. We show that using this fibre assembly, a simple and robust pulsed laser delivery system without any free-space optics, which is thus suitable for clinical use, can be realised.
Original languageEnglish
Title of host publicationProceedings of SPIE
Number of pages8
PublisherSPIE - International Society for Optical Engineering
Publication date2018
DOIs
Publication statusPublished - 2018
EventSPIE Photonics West 2018: Multiphoton Microscopy in the Biomedical Sciences XVIII - Moscone Center, San Francisco, United States
Duration: 27 Jan 20181 Feb 2018
Conference number: 10498
https://spie.org/PWB/conferencedetails/multiphoton-microscopy

Conference

ConferenceSPIE Photonics West 2018
Number10498
LocationMoscone Center
Country/TerritoryUnited States
CitySan Francisco
Period27/01/201801/02/2018
Internet address
SeriesProceedings of SPIE, the International Society for Optical Engineering
Volume10498
ISSN0277-786X

Keywords

  • Two-photon microscopy
  • Femtosecond laser pulse
  • Dispersion management

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