Simple fibre based dispersion management for two-photon excited fluorescence imaging through an endoscope

Konstantinos Dimopoulos; Dominik Marti; Peter E. Andersen

doi:10.1117/12.2289533

Simple fibre based dispersion management for two-photon excited fluorescence imaging through an endoscope

Konstantinos Dimopoulos, Dominik Marti, Peter E. Andersen

Copenhagen Center for Health Technology

Technical University of Denmark

Research output: Chapter in Book/Report/Conference proceeding › Article in proceedings › Research › peer-review

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Abstract

We want to implement two-photon excitation fluorescence microscopy (TPEFM) into endoscopes, since TPEFM can provide relevant biomarkers for cancer staging and grading in hollow organs, endoscopically accessible through natural orifices. However, many obstacles must be overcome, among others the delivery of short laser
pulses to the distal end of the endoscope. To this avail, we present imaging results using an all-fibre dispersion management scheme in a TPEFM setup. The scheme has been conceived by Jespersen et al. in 20101 and relies on the combination of a single mode fibre with normal and a higher order mode fibre with anomalous dispersion properties, fused in series using a long period grating. We show that using this fibre assembly, a simple and robust pulsed laser delivery system without any free-space optics, which is thus suitable for clinical use, can be realised.

Original language	English
Title of host publication	Proceedings of SPIE
Number of pages	8
Publisher	SPIE - International Society for Optical Engineering
Publication date	2018
DOIs	https://doi.org/10.1117/12.2289533
Publication status	Published - 2018
Event	SPIE Photonics West 2018: Multiphoton Microscopy in the Biomedical Sciences XVIII - Moscone Center, San Francisco, United States Duration: 27 Jan 2018 → 1 Feb 2018 Conference number: 10498 https://spie.org/PWB/conferencedetails/multiphoton-microscopy

Conference

Conference	SPIE Photonics West 2018
Number	10498
Location	Moscone Center
Country/Territory	United States
City	San Francisco
Period	27/01/2018 → 01/02/2018
Internet address	https://spie.org/PWB/conferencedetails/multiphoton-microscopy

Series	Proceedings of SPIE - The International Society for Optical Engineering
ISSN	0277-786X

Keywords

Two-photon microscopy
Femtosecond laser pulse
Dispersion management

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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MIB: Multi-modal, Endoscopic Biophotonic Imaging of Bladder Cancer for Point-of-Care Diagnosis
Marti, D., Hansen, A. K., Jensen, O. B. & Andersen, P. E.
01/01/2016 → 31/12/2021
Project: Research

Cite this

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abstract = "We want to implement two-photon excitation fluorescence microscopy (TPEFM) into endoscopes, since TPEFM can provide relevant biomarkers for cancer staging and grading in hollow organs, endoscopically accessible through natural orifices. However, many obstacles must be overcome, among others the delivery of short laserpulses to the distal end of the endoscope. To this avail, we present imaging results using an all-fibre dispersion management scheme in a TPEFM setup. The scheme has been conceived by Jespersen et al. in 20101 and relies on the combination of a single mode fibre with normal and a higher order mode fibre with anomalous dispersion properties, fused in series using a long period grating. We show that using this fibre assembly, a simple and robust pulsed laser delivery system without any free-space optics, which is thus suitable for clinical use, can be realised.",

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note = "SPIE Photonics West 2018 : Multiphoton Microscopy in the Biomedical Sciences XVIII ; Conference date: 27-01-2018 Through 01-02-2018",

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Dimopoulos, K, Marti, D & Andersen, PE 2018, Simple fibre based dispersion management for two-photon excited fluorescence imaging through an endoscope. in Proceedings of SPIE. SPIE - International Society for Optical Engineering, Proceedings of SPIE - The International Society for Optical Engineering, SPIE Photonics West 2018, San Francisco, California, United States, 27/01/2018. https://doi.org/10.1117/12.2289533

Simple fibre based dispersion management for two-photon excited fluorescence imaging through an endoscope. / Dimopoulos, Konstantinos; Marti, Dominik; Andersen, Peter E.

Proceedings of SPIE. SPIE - International Society for Optical Engineering, 2018. (Proceedings of SPIE - The International Society for Optical Engineering).

Research output: Chapter in Book/Report/Conference proceeding › Article in proceedings › Research › peer-review

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AB - We want to implement two-photon excitation fluorescence microscopy (TPEFM) into endoscopes, since TPEFM can provide relevant biomarkers for cancer staging and grading in hollow organs, endoscopically accessible through natural orifices. However, many obstacles must be overcome, among others the delivery of short laserpulses to the distal end of the endoscope. To this avail, we present imaging results using an all-fibre dispersion management scheme in a TPEFM setup. The scheme has been conceived by Jespersen et al. in 20101 and relies on the combination of a single mode fibre with normal and a higher order mode fibre with anomalous dispersion properties, fused in series using a long period grating. We show that using this fibre assembly, a simple and robust pulsed laser delivery system without any free-space optics, which is thus suitable for clinical use, can be realised.

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Simple fibre based dispersion management for two-photon excited fluorescence imaging through an endoscope

Abstract

Conference

Keywords

UN SDGs

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MIB: Multi-modal, Endoscopic Biophotonic Imaging of Bladder Cancer for Point-of-Care Diagnosis

Cite this