Simple DPPHC-Based Electrochemical Assay for the Evaluation of the Antioxidant Capacity: a Thorough Comparison with Spectrophotometric Assays and Evaluation with Real-World Samples

Veronica Andrei; Ada-Ioana Bunea; Aurelia  Tudorache; Szilveszter  Gáspár; Alina Vasilescu

doi:10.1002/elan.201400376

Simple DPPHC-Based Electrochemical Assay for the Evaluation of the Antioxidant Capacity: a Thorough Comparison with Spectrophotometric Assays and Evaluation with Real-World Samples

Veronica Andrei, Ada-Ioana Bunea, Aurelia Tudorache, Szilveszter Gáspár, Alina Vasilescu

Research output: Contribution to journal › Journal article › Research › peer-review

Abstract

An assay based on the electrochemical detection of 2,2-diphenyl-1-picrylhydrazyl radical (DPPHC) for the evaluation of the total antioxidant capacity (TAC) was optimized. The assay is interchangeable with the classic spectrophotometric tests for TAC based on the same radical. In addition, it can be used for the analysis of
dilute samples with low antioxidant capacities. A good linear correlation (R2=0.97) was obtained between the results obtained with the proposed electrochemical assay
and the Trolox Equivalent Antioxidant Capacity test based on ABTS radical. The assay was successfully used to evaluate the antioxidant capacity of two red wines obtained by six different maceration-fermentation techniques.

Original language	English
Journal	Electroanalysis
Volume	14
Number of pages	10
ISSN	1040-0397
DOIs	https://doi.org/10.1002/elan.201400376
Publication status	Published - 2014
Externally published	Yes

Keywords

Total antioxidant capacity
DPPH
Amperometry
Flow injection analysis
Wine

Access to Document

10.1002/elan.201400376

OpenUrl availability

Full text

Cite this

@article{94bf607c0610473abea520e42ff4025e,

title = "Simple DPPHC-Based Electrochemical Assay for the Evaluation of the Antioxidant Capacity: a Thorough Comparison with Spectrophotometric Assays and Evaluation with Real-World Samples",

abstract = "An assay based on the electrochemical detection of 2,2-diphenyl-1-picrylhydrazyl radical (DPPHC) for the evaluation of the total antioxidant capacity (TAC) was optimized. The assay is interchangeable with the classic spectrophotometric tests for TAC based on the same radical. In addition, it can be used for the analysis ofdilute samples with low antioxidant capacities. A good linear correlation (R2=0.97) was obtained between the results obtained with the proposed electrochemical assayand the Trolox Equivalent Antioxidant Capacity test based on ABTS radical. The assay was successfully used to evaluate the antioxidant capacity of two red wines obtained by six different maceration-fermentation techniques.",

keywords = "Total antioxidant capacity, DPPH, Amperometry, Flow injection analysis, Wine",

author = "Veronica Andrei and Ada-Ioana Bunea and Aurelia Tudorache and Szilveszter G{\'a}sp{\'a}r and Alina Vasilescu",

year = "2014",

doi = "10.1002/elan.201400376",

language = "English",

volume = "14",

journal = "Electroanalysis",

issn = "1040-0397",

publisher = "Wiley - V C H Verlag GmbH & Co. KGaA",

}

Simple DPPHC-Based Electrochemical Assay for the Evaluation of the Antioxidant Capacity: a Thorough Comparison with Spectrophotometric Assays and Evaluation with Real-World Samples. / Andrei, Veronica; Bunea, Ada-Ioana; Tudorache, Aurelia et al.
In: Electroanalysis, Vol. 14, 2014.

Research output: Contribution to journal › Journal article › Research › peer-review

TY - JOUR

T1 - Simple DPPHC-Based Electrochemical Assay for the Evaluation of the Antioxidant Capacity: a Thorough Comparison with Spectrophotometric Assays and Evaluation with Real-World Samples

AU - Andrei, Veronica

AU - Bunea, Ada-Ioana

AU - Tudorache, Aurelia

AU - Gáspár, Szilveszter

AU - Vasilescu, Alina

PY - 2014

Y1 - 2014

N2 - An assay based on the electrochemical detection of 2,2-diphenyl-1-picrylhydrazyl radical (DPPHC) for the evaluation of the total antioxidant capacity (TAC) was optimized. The assay is interchangeable with the classic spectrophotometric tests for TAC based on the same radical. In addition, it can be used for the analysis ofdilute samples with low antioxidant capacities. A good linear correlation (R2=0.97) was obtained between the results obtained with the proposed electrochemical assayand the Trolox Equivalent Antioxidant Capacity test based on ABTS radical. The assay was successfully used to evaluate the antioxidant capacity of two red wines obtained by six different maceration-fermentation techniques.

AB - An assay based on the electrochemical detection of 2,2-diphenyl-1-picrylhydrazyl radical (DPPHC) for the evaluation of the total antioxidant capacity (TAC) was optimized. The assay is interchangeable with the classic spectrophotometric tests for TAC based on the same radical. In addition, it can be used for the analysis ofdilute samples with low antioxidant capacities. A good linear correlation (R2=0.97) was obtained between the results obtained with the proposed electrochemical assayand the Trolox Equivalent Antioxidant Capacity test based on ABTS radical. The assay was successfully used to evaluate the antioxidant capacity of two red wines obtained by six different maceration-fermentation techniques.

KW - Total antioxidant capacity

KW - DPPH

KW - Amperometry

KW - Flow injection analysis

KW - Wine

U2 - 10.1002/elan.201400376

DO - 10.1002/elan.201400376

M3 - Journal article

SN - 1040-0397

VL - 14

JO - Electroanalysis

JF - Electroanalysis

ER -

Simple DPPHC-Based Electrochemical Assay for the Evaluation of the Antioxidant Capacity: a Thorough Comparison with Spectrophotometric Assays and Evaluation with Real-World Samples

Abstract

Keywords

Access to Document

OpenUrl availability

Fingerprint

Cite this