Abstract
In this study, antibacterial characteristic of silver/poly (lactic acid) nanocomposite (Ag/PLA-NC) films was investigated, while silver nanoparticles (Ag-NPs) were synthesized into biodegradable PLA via chemical reduction method in diphase solvent. Silver nitrate and sodium borohydride were respectively used as a silver precursor and reducing agent in the PLA, which acted as a polymeric matrix and stabilizer. Meanwhile, the properties of Ag/PLA-NCs were studied as a function of the Ag-NP weight percentages (8, 16, and 32 wt% respectively), in relation to the use of PLA. The morphology of the Ag/PLA-NC films and the distribution of the Ag-NPs were also characterized. The silver ions released from the Ag/PLA-NC films and their antibacterial activities were scrutinized. The antibacterial activities of the Ag/PLA-NC films were examined against Gram-negative bacteria (Escherichia coli and Vibrio parahaemolyticus) and Gram-positive bacteria (Staphylococcus aureus) by diffusion method using Muller-Hinton agar. The results indicated that Ag/PLA-NC films possessed a strong antibacterial activity with the increase in the percentage of Ag-NPs in the PLA. Thus, Ag/PLA-NC films can be used as an antibacterial scaffold for tissue engineering and medical application. �� 2010 Shameli et al, publisher and licensee Dove Medical Press Ltd.
| Original language | English |
|---|---|
| Journal | International Journal of Nanomedicine (Online) |
| Volume | 5 |
| Issue number | 1 |
| Pages (from-to) | 573-579 |
| ISSN | 1176-9114 |
| Publication status | Published - 2010 |
| Externally published | Yes |
Bibliographical note
© 2010 Shameli et al, publisher and licensee Dove Medical Press Ltd. This is an Open Access article which permits unrestricted noncommercial use, provided the original work is properly cited.Keywords
- Biophysics
- Bioengineering
- Biomaterials
- Organic Chemistry
- Drug Discovery
- Antibacterial activity
- Biodegradable
- Muller-Hinton agar
- Poly (lactic acid)
- Silver nanoparticles
- lactic acid
- molecular scaffold
- silver nanoparticle
- sodium borohydride
- antiinfective agent
- metal nanoparticle
- nanocomposite
- silver
- tissue scaffold
- agar diffusion
- antibacterial activity
- article
- biodegradability
- chemical structure
- controlled study
- Escherichia coli
- infrared spectroscopy
- materials testing
- molecular weight
- nonhuman
- reduction kinetics
- solvent effect
- Staphylococcus aureus
- tissue engineering
- ultraviolet spectroscopy
- Vibrio parahaemolyticus
- X ray diffraction
- chemistry
- drug effect
- microbiological examination
- nanomedicine
- particle size
- spectrophotometry
- transmission electron microscopy
- ultrastructure
- Negibacteria
- Posibacteria
- Anti-Bacterial Agents
- Metal Nanoparticles
- Microbial Sensitivity Tests
- Microscopy, Electron, Transmission
- Nanocomposites
- Nanomedicine
- Particle Size
- Silver
- Spectrophotometry
- Spectroscopy, Fourier Transform Infrared
- Tissue Scaffolds
- X-Ray Diffraction