Silver nanoparticles produced from Cedecea sp. exhibit antibiofilm activity and remarkable stability

Priyanka Singh, Santosh Pandit, Carsten Jers, Abhayraj S. Joshi, Jørgen Garnæs, Ivan Mijakovic*

*Corresponding author for this work

Research output: Contribution to journalJournal articleResearchpeer-review

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Abstract

With multidrug-resistant bacterial pathogens on the rise, there is a strong research focus on alternative antibacterial treatments that could replace or complement classical antibiotics. Metallic nanoparticles, and in particular silver nanoparticles (AgNPs), have been shown to kill bacterial biofilms effectively, but their chemical synthesis often involves environmentally unfriendly by-products. Recent studies have shown that microbial and plant extracts can be used for the environmentally friendly synthesis of AgNPs. Herein we report a procedure for producing AgNPs using a putative Cedecea sp. strain isolated from soil. The isolated bacterial strain showed a remarkable potential for producing spherical, crystalline and stable AgNPs characterized by UV–visible spectroscopy, transmission electron microscopy, dynamic light scattering, and Fourier transform infrared spectroscopy. The concentration of produced nanoparticles was 1.31 µg/µl with a negative surface charge of − 15.3 mV and nanoparticles size ranging from 10–40 nm. The AgNPs was tested against four pathogenic microorganisms S. epidermidis, S. aureus, E. coli and P. aeruginosa. The nanoparticles exhibited strong minimum inhibitory concentration (MIC) values of 12.5 and 6.25 µg/µl and minimum bactericidal concentration (MBC) values of 12.5 and 12.5 µg/mL against E. coli and P. aeruginosa, respectively. One distinguishing feature of AgNPs produced by Cedecea sp. extracts is their extreme stability. Inductively coupled plasma mass spectrometry and thermogravimetric analysis demonstrated that the produced AgNPs are stable for periods exceeding one year. This means that their strong antibacterial effects, demonstrated against E. coli and P. aeruginosa biofilms, can be expected to persist during extended periods.

Original languageEnglish
Article number12619
JournalScientific Reports
Volume11
Issue number1
Number of pages13
ISSN2045-2322
DOIs
Publication statusPublished - 2021

Bibliographical note

Funding Information:
Lundbeckfonden to PS (R303-2018-3499), Novo Nordisk Foundation (NNF10CC1016517) and the Independent Research Fund Denmark–FTP to IM funded the study. ICPMS was performed at DTU Environmental; TEM was performed at Center for electron microscopy, DTU and FTIR was conducted at Department of Chemical Engineering, DTU. Funds support AFMs contribution from The Danish Agency for Institutions and Educational Grants.

Publisher Copyright:
© 2021, The Author(s).

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