Short time window for transmissibility of African swine fever virus from a contaminated environment

A S Olesen, L Lohse, A. Boklund, T. Halasa, G. J. Belsham, T. B. Rasmussen, A. Bøtner*

*Corresponding author for this work

    Research output: Contribution to journalJournal articleResearchpeer-review

    Abstract

    Since the introduction of African swine fever virus (ASFV) into the Baltic states and Poland in 2014, the disease has continued to spread within these regions. In 2017, the virus spread further west and the first cases of disease were reported in the Czech Republic and Romania, in wild boar and domestic pigs, respectively. To control further spread, knowledge of different modes of transmission, including indirect transmission via a contaminated environment, is crucial. Up until now, such an indirect mode of transmission has not been demonstrated. In this study, transmission via an environment contaminated with excretions from ASFV-infected pigs was investigated. Following euthanasia of pigs that were infected with an isolate of ASFV from Poland (POL/2015/Podlaskie/Lindholm), healthy pigs were introduced into the pens, in which the ASFV-infected pigs had been housed. Introduction was performed at 1, 3, 5 or 7 days, following euthanasia of the infected pig groups. Pigs, that were introduced into the contaminated environment after 1 day, developed clinical disease within 1 week, and both ASFV DNA and infectious virus were isolated from their blood. However, pigs introduced into the contaminated pens after 3, 5 or 7 days did not develop any signs of ASFV infection and no viral DNA was detected in blood samples obtained from these pigs within the following 3 weeks. Thus, it was shown that exposure of pigs to an environment contaminated with ASFV can result in infection. However, the time window for transmissibility of ASFV seems very limited, and, within our experimental system, there appears to be a rapid decrease in the infectivity of ASFV in the environment.
    Original languageEnglish
    JournalTransboundary and Emerging Diseases
    Volume65
    Issue number4
    Pages (from-to)1024-1032
    ISSN1865-1674
    DOIs
    Publication statusPublished - 2018

    Keywords

    • African swine fever virus
    • Environment
    • Fomite
    • Haemorrhagic disease
    • Virus transmission

    Cite this

    Olesen, A. S., Lohse, L., Boklund, A., Halasa, T., Belsham, G. J., Rasmussen, T. B., & Bøtner, A. (2018). Short time window for transmissibility of African swine fever virus from a contaminated environment. Transboundary and Emerging Diseases, 65(4), 1024-1032. https://doi.org/10.1111/tbed.12837