Short-term time course of liver metabolic response to acute handling stress in rainbow trout, Oncorhynchus mykiss

Marcos A. Lopez-Patino, Juan Hernandez-Perez, Manuel Gesto, Marta Libran-Perez, Jesus M. Miguez, Jose L. Soengas

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

To elucidate the short-term time-course of liver metabolic response in rainbow trout to acute handling stress we subjected rainbow trout to 5 mm chasing and obtained samples 0 to 480 mm post-stress. Levels of cortisol, glucose and lactate were measured in plasma, whereas metabolite levels, enzyme activities, mRNA abundance of parameters related to energy metabolism, and glucocorticoid receptors were assessed in liver. Acute stress affected many parameters related to energy metabolism, with most of them turning back to normal levels after 480 mm. In general, the present results support the existence of two stages in the short-term time-course of metabolic response to handling stress. A first stage occurring few minutes post-stress (15-45 min), was characterized by increased mobilization of liver glycogen resulting in increased production of endogenous glucose, reduced use of exogenous glucose and reduced lipogenic potential. A second stage, occurring 60-120 mm post-stress onwards was characterized by the recovery of liver glycogen levels, the increased capacity of liver for releasing glucose, and the recovery of lipogenic capacity whereas no changes were noted in gluconeogenic potential, which probably needs longer time periods to become enhanced. (C) 2013 Elsevier Inc. All rights reserved.
Original languageEnglish
JournalComparative Biochemistry and Physiology - Part A: Molecular & Integrative Physiology
Volume168A
Pages (from-to)40-49
ISSN1095-6433
DOIs
Publication statusPublished - 2014
Externally publishedYes

Keywords

  • BIOCHEMISTRY
  • PHYSIOLOGY
  • ZOOLOGY
  • BROCKMANN BODIES
  • ENERGY-METABOLISM
  • SPARUS-AURATA
  • FOOD-INTAKE
  • GLUCOCORTICOID-RECEPTOR
  • GLUCOSENSING CAPACITY
  • GLYCOGEN-METABOLISM
  • CORTISOL RESPONSE
  • GENE-EXPRESSION
  • GROWTH-HORMONE
  • Rainbow trout
  • Liver
  • Handling
  • Time-course
  • Energy metabolism
  • Biochemistry
  • Molecular Biology
  • Physiology
  • glucocorticoid receptor
  • glucose
  • hydrocortisone
  • lactic acid
  • messenger RNA
  • acute stress
  • animal experiment
  • article
  • controlled study
  • energy metabolism
  • enzyme activity
  • enzyme immobilization
  • glucose blood level
  • glycogen liver level
  • hydrocortisone blood level
  • lactate blood level
  • lipid metabolism
  • liver metabolism
  • nonhuman
  • nucleotide sequence
  • rainbow trout
  • Oncorhynchus mykiss
  • Acetyl-CoA C-Acetyltransferase
  • Animal Husbandry
  • Animals
  • Aquaculture
  • Blood Glucose
  • Carboxy-Lyases
  • Fatty Acid Synthases
  • Fatty Acids
  • Fish Proteins
  • Gene Expression
  • Gene Expression Regulation, Enzymologic
  • Glucokinase
  • Glucose-6-Phosphatase
  • Glycogen
  • Glycogen Phosphorylase
  • Glycogen Synthase
  • Hydrocortisone
  • Lactic Acid
  • Metabolic Networks and Pathways
  • Pyruvate Kinase
  • Stress, Physiological
  • Triglycerides
  • Pisces Vertebrata Chordata Animalia (Animals, Chordates, Fish, Nonhuman Vertebrates, Vertebrates) - Osteichthyes [85206] Oncorhynchus mykiss species rainbow trout common
  • carbohydrates
  • cortisol 50-23-7 metabolism
  • glucose 58367-01-4 metabolism
  • glycogen 9005-79-2
  • lactate 113-21-3 metabolism
  • 10060, Biochemistry studies - General
  • 10062, Biochemistry studies - Nucleic acids, purines and pyrimidines
  • 10067, Biochemistry studies - Sterols and steroids
  • 10068, Biochemistry studies - Carbohydrates
  • 10802, Enzymes - General and comparative studies: coenzymes
  • 13002, Metabolism - General metabolism and metabolic pathways
  • 14004, Digestive system - Physiology and biochemistry
  • 15002, Blood - Blood and lymph studies
  • 15004, Blood - Blood cell studies
  • Biochemistry and Molecular Biophysics
  • liver digestive system
  • plasma blood and lymphatics
  • Enzymology
  • Metabolism

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