Shared strategies for β-lactam catabolism in the soil microbiome

Research output: Contribution to journalJournal article – Annual report year: 2018Researchpeer-review

Documents

DOI

  • Author: Crofts, Terence S.

    Washington University St. Louis, United States

  • Author: Wang, Bin

    Washington University St. Louis, United States

  • Author: Spivak, Aaron

    Washington University St. Louis, United States

  • Author: Gianoulis, Tara A.

    Harvard University, United States

  • Author: Forsberg, Kevin J.

    Washington University St. Louis, United States

  • Author: Gibson, Molly K.

    Washington University St. Louis, United States

  • Author: Johnsky, Lauren A.

    Edgewood Chemical Biological Center, United States

  • Author: Broomall, Stacey M.

    Edgewood Chemical Biological Center, United States

  • Author: Rosenzweig, C. Nicole

    Edgewood Chemical Biological Center, United States

  • Author: Skowronski, Evan W.

    Edgewood Chemical Biological Center, United States

  • Author: Gibbons, Henry S.

    Edgewood Chemical Biological Center, United States

  • Author: Sommer, Morten O. A.

    Bacterial Synthetic Biology, Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Kemitorvet, 2800, Kgs. Lyngby, Denmark

  • Author: Dantas, Gautam

    Washington University St. Louis, United States

View graph of relations

The soil microbiome can produce, resist, or degrade antibiotics and even catabolize them. While resistance genes are widely distributed in the soil, there is a dearth of knowledge concerning antibiotic catabolism. Here we describe a pathway for penicillin catabolism in four isolates. Genomic and transcriptomic sequencing revealed β-lactamase, amidase, and phenylacetic acid catabolon upregulation. Knocking out part of the phenylacetic acid catabolon or an apparent penicillin utilization operon (put) resulted in loss of penicillin catabolism in one isolate. A hydrolase from the put operon was found to degrade in vitro benzylpenicilloic acid, the β-lactamase penicillin product. To test the generality of this strategy, an Escherichia coli strain was engineered to co-express a β-lactamase and a penicillin amidase or the put operon, enabling it to grow using penicillin or benzylpenicilloic acid, respectively. Elucidation of additional pathways may allow bioremediation of antibiotic-contaminated soils and discovery of antibiotic-remodeling enzymes with industrial utility.
Original languageEnglish
JournalNature Chemical Biology
Volume14
Pages (from-to)556-564
ISSN1552-4450
DOIs
Publication statusPublished - 2018
CitationsWeb of Science® Times Cited: No match on DOI

Download statistics

No data available

ID: 148212899