Sex-specific expression of a novel gene Tmem184a during mouse testis differentiation

Terje Svingen, Annemiek Beverdam, Pascal Bernard, Peter McClive, Vincent R. Harley, Andrew H. Sinclair, Peter Koopman

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

During mouse embryogenesis, the fate of the bipotential gonads is sealed around 10.5 days post coitum (dpc) when the Y-linked gene Sry specifies the differentiation of testes in males, whereas in females, absence of Sry results in ovary formation. Apart from the pivotal action of Sry, many other genes are known to be involved in sex determination and subsequent differentiation. Much is still unknown regarding the regulatory hierarchy governing these events and many more sex differentiation genes are yet to be discovered. In this study, we investigated the expression of Tmem184a, a novel gene encoding a protein of unknown function, but with predicted kinase activity, during mouse embryogenesis. We show that TrnemI84a is expressed at high levels in the developing testis from 11.5 dpc, a time of active proliferation and differentiation. Trnem184a expression is further shown to be expressed exclusively within the Sertoli cells of the developing testis cords, suggesting that it may mediate sex-specific signaling events during Sertoli cell differentiation.
Original languageEnglish
JournalReproduction
Volume133
Issue number5
Pages (from-to)983-989
Number of pages7
ISSN1470-1626
DOIs
Publication statusPublished - 2007
Externally publishedYes

Keywords

  • Animals
  • Cell Differentiation
  • Embryo, Mammalian
  • Gene Expression
  • Gene Expression Regulation, Developmental
  • Gestational Age
  • In Situ Hybridization
  • Male
  • Membrane Proteins
  • Mice
  • Reverse Transcriptase Polymerase Chain Reaction
  • Sertoli Cells
  • Sex Differentiation
  • Testis
  • Tmem184a protein, mouse
  • membrane protein
  • protein Tmem184a
  • unclassified drug
  • animal cell
  • animal experiment
  • animal tissue
  • article
  • cell differentiation
  • cell proliferation
  • embryo
  • embryo development
  • enzyme activity
  • fetus
  • male
  • mouse
  • nonhuman
  • nucleotide sequence
  • priority journal
  • protein expression
  • protein function
  • protein localization
  • Sertoli cell
  • sex differentiation
  • SRY gene
  • testis development
  • tissue specificity
  • Embryo
  • DEVELOPMENTAL
  • REPRODUCTIVE
  • SERTOLI-CELLS
  • DETERMINATION REVEALS
  • SOX9
  • MICE
  • SRY
  • MIGRATION
  • EMBRYOS
  • XY
  • embryogenesis
  • sex determination
  • Rodentia Mammalia Vertebrata Chordata Animalia (Animals, Chordates, Mammals, Nonhuman Vertebrates, Nonhuman Mammals, Rodents, Vertebrates) - Muridae [86375] mouse common embryo
  • mouse Sry gene [Muridae]
  • mouse Tmem184a gene [Muridae] expression
  • 02506, Cytology - Animal
  • 03502, Genetics - General
  • 03506, Genetics - Animal
  • 16504, Reproductive system - Physiology and biochemistry
  • 17002, Endocrine - General
  • 17006, Endocrine - Gonads and placenta
  • 25502, Development and Embryology - General and descriptive
  • Biochemistry and Molecular Biophysics
  • Chemical Coordination and Homeostasis
  • Reproduction
  • gonad reproductive system, endocrine system
  • ovary reproductive system
  • Sertoli cell reproductive system
  • testis reproductive system
  • Endocrine System
  • Molecular Genetics
  • Reproductive System

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