Serotyping of Actinobacillus pleuropneumoniae serotype 5 strains using a monoclonal-based polystyrene agglutination test

J.D. Dubreuil, A. Letellier, Eva Stenbæk, M. Gottschalk

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    Abstract

    A polystyrene agglutination test has been developed for serotyping Actinobacillus pleuropneumoniae serotype 5a and 5b strains. Protein A-coated polystyrene microparticles were sensitized with a murine monoclonal antibody recognizing an epitope on serotype 5 LPS-O chain as shown by SDS-PAGE and Western blotting, A total of 205 A. pleuropneumoniae, strains including all 12 serotype reference strains and 13 strains representing 8 common bacterial species associated with swine or related to A, pleuropneumoniae, were tested by mixing 25 mu L of polystyrene reagent with the same volume of a dense suspension of bacterial cells grown for 18 h. All A, pleuropneumoniae strains had been previously serotyped using standard procedures, The polystyrene agglutination test was rapid (less than 3 min) and easy to perform. Overall a very good correlation (97.3%) with the standard techniques was found, The sensitized polystyrene particles were stable for at least 6 mo.
    Original languageEnglish
    JournalCanadian Journal of Veterinary Research-Revue Canadienne de Recherche Veterinaire
    Volume60
    Issue number1
    Pages (from-to)69-71
    ISSN0830-9000
    Publication statusPublished - 1996

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