Serological discrimination by indirect enzyme immunoassay between the antibody response to Brucella sp and Yersinia enterocolitica O : 9 in cattle and pigs

K. Nielsen, P. Smith, W. Yu, P. Nicoletti, Gregers Jungersen, J. Stack, J. Godfroid

    Research output: Contribution to journalJournal articleResearchpeer-review

    Abstract

    A rapid, inexpensive and rugged serological test that distinguishes cattle and swine infected with Brucella sp. or Yersinia enterocolitica O:9 is described. The test protocol, which is an indirect enzyme immunoassay uses a high concentration of divalent cation chelating agents to minimize binding of Y. enterocolitica O:9 antibody to rough lipopolysaccharide antigen derived from B. abortus RB51. No false positive reactions were observed when testing 100 Canadian cattle and swine without any evidence of brucellosis. The assay detected 91.6% of cattle (n = 155) and 93.5% (n = 31) of swine infected with Brucella sp. Sera from 58 cattle and 38 swine exposed to Y. enterocolitica O:9 were negative while only 20 sera from 121 'false positive' reactors of unspecified origin gave low level positive reactions, eliminating 84% of the false positive reactions. Crown
    Original languageEnglish
    JournalVeterinary Immunology and Immunopathology
    Volume109
    Issue number1-2
    Pages (from-to)69-78
    ISSN0165-2427
    DOIs
    Publication statusPublished - 2006

    Keywords

    • differentiation
    • Yersinia enterocolitica O : 9
    • Brucella sp.
    • serology
    • IELISA

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