TY - JOUR
T1 - Serological comparison of systemic lupus erythematosus with neuropsychiatric lupus using synthetic nucleic acid antigens
AU - Khatri, Sangita
AU - Psaraftis, Nikos
AU - Funaro, Alessia
AU - Arinuma, Yoshiyuki
AU - Fujieda, Yuichiro
AU - Mader, Simone
AU - Jørgensen, Christian Damsgaard
AU - Astakhova, Kira
PY - 2020
Y1 - 2020
N2 - Neuropsychiatric systemic lupus erythematosus is an autoimmune disorder characterized by an irregular exchange between the central nervous system and the immune system, leading to the outbreak of neurological conditions with possible disabling effects. Although neuropsychiatric systemic lupus erythematosus is the most common expression of lupus condition, it is still poorly understood. In this study, we focus on the development of an advantageous method based on the application of synthetic nucleic acids and protein-based antigen arrays in order to characterize autoreactive antibodies in neuropsychiatric systemic lupus erythematosus. We confirmed the benefits of using synthetic oligonucleotides such as assay reproducibility, elevated affinity and specificity to autoreactive antibodies. We also demonstrated presence of autoantibodies towards three particular synthetic double stranded antigens and verify similarity of antinuclear antibody patterns in ordinary lupus and neuropsychiatric systemic lupus erythematosus.
AB - Neuropsychiatric systemic lupus erythematosus is an autoimmune disorder characterized by an irregular exchange between the central nervous system and the immune system, leading to the outbreak of neurological conditions with possible disabling effects. Although neuropsychiatric systemic lupus erythematosus is the most common expression of lupus condition, it is still poorly understood. In this study, we focus on the development of an advantageous method based on the application of synthetic nucleic acids and protein-based antigen arrays in order to characterize autoreactive antibodies in neuropsychiatric systemic lupus erythematosus. We confirmed the benefits of using synthetic oligonucleotides such as assay reproducibility, elevated affinity and specificity to autoreactive antibodies. We also demonstrated presence of autoantibodies towards three particular synthetic double stranded antigens and verify similarity of antinuclear antibody patterns in ordinary lupus and neuropsychiatric systemic lupus erythematosus.
KW - Autoreactive antibodies
KW - Enzyme-Linked Immunosorbent assay
KW - Neuropsychiatric systemic lupus erythematosus
KW - Oligonucleotides
KW - Synthetic Antigens
U2 - 10.1016/j.jtauto.2020.100068
DO - 10.1016/j.jtauto.2020.100068
M3 - Journal article
C2 - 33205038
AN - SCOPUS:85095428816
SN - 2589-9090
VL - 3
JO - Journal of Translational Autoimmunity
JF - Journal of Translational Autoimmunity
M1 - 100068
ER -