Serological comparison of systemic lupus erythematosus with neuropsychiatric lupus using synthetic nucleic acid antigens

Sangita Khatri, Nikos Psaraftis, Alessia Funaro, Yoshiyuki Arinuma, Yuichiro Fujieda, Simone Mader, Christian Damsgaard Jørgensen, Kira Astakhova*

*Corresponding author for this work

Research output: Contribution to journalJournal articleResearchpeer-review

11 Downloads (Pure)

Abstract

Neuropsychiatric systemic lupus erythematosus is an autoimmune disorder characterized by an irregular exchange between the central nervous system and the immune system, leading to the outbreak of neurological conditions with possible disabling effects. Although neuropsychiatric systemic lupus erythematosus is the most common expression of lupus condition, it is still poorly understood. In this study, we focus on the development of an advantageous method based on the application of synthetic nucleic acids and protein-based antigen arrays in order to characterize autoreactive antibodies in neuropsychiatric systemic lupus erythematosus. We confirmed the benefits of using synthetic oligonucleotides such as assay reproducibility, elevated affinity and specificity to autoreactive antibodies. We also demonstrated presence of autoantibodies towards three particular synthetic double stranded antigens and verify similarity of antinuclear antibody patterns in ordinary lupus and neuropsychiatric systemic lupus erythematosus.

Original languageEnglish
Article number100068
JournalJournal of Translational Autoimmunity
Volume3
Number of pages8
DOIs
Publication statusPublished - 2020

Keywords

  • Autoreactive antibodies
  • Enzyme-Linked Immunosorbent assay
  • Neuropsychiatric systemic lupus erythematosus
  • Oligonucleotides
  • Synthetic Antigens

Fingerprint Dive into the research topics of 'Serological comparison of systemic lupus erythematosus with neuropsychiatric lupus using synthetic nucleic acid antigens'. Together they form a unique fingerprint.

Cite this