Sequestration of zinc oxide by fimbrial designer chelators

Kristian Kjærgaard, Jack K Sørensen, Mark Schembri, Per Klemm

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

Type 1 fimbriae are surface organelles of Escherichia coli. By engineering a structural component of the fimbriae, FimH, to display a random peptide library, we were able to isolate metal-chelating bacteria. A library consisting of 4 x 10(7) independent clones was screened for binding to ZnO. Sequences responsible for ZnO adherence were identified, and distinct binding motifs were characterized. The sequences selected exhibited various degrees of affinity and specificity towards ZnO. Competitive binding experiments revealed that the sequences recognized only the oxide form of Zn. Interestingly, one of the inserts exhibited significant homology to a specific sequence in a putative zinc-containing helicase, which suggests that searches such as this one may aid in identifying binding motifs in nature. The zinc-binding bacteria might have a use in detoxification of metal-polluted water
Original languageEnglish
JournalAPPLIED AND ENVIRONMENTAL MICROBIOLOGY
Volume66
Issue number1
Pages (from-to)10-14
Publication statusPublished - 2000

Cite this

Kjærgaard, K., Sørensen, J. K., Schembri, M., & Klemm, P. (2000). Sequestration of zinc oxide by fimbrial designer chelators. APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 66(1), 10-14.