A sequential injection analysis system is described that incorporates a nylon tubular reactor containing immobilised glucose oxidase, allowing determination of D-glucose by means of subsequent luminol chemiluminescence detection of the hydrogen peroxide generated in the enzymatic reaction. The operating parameters were optimised by fractional factorial screening and response surface modelling. The linear range of D-glucose determination was 30-600 mu M, With a detection limit of 15 mu M using a photodiode detector. The sampling frequency was 54 h(-1). Lower LOD (0.5 mu M D-glucose) could be reached by using a PMT as the detector. Fermentation broth samples were determined and good recoveries were obtained.
|Journal||Analytica Chimica Acta|
|Publication status||Published - 1996|
|Event||Fifth National Conference on Flow Injection Analysis - Qindao, China|
Duration: 1 Jan 1996 → …
|Conference||Fifth National Conference on Flow Injection Analysis|
|Period||01/01/1996 → …|