Sequential Injection Determination of D-Glucose by Chemiluminescence Using an Open Tubular Immobilised Enzyme Reactor

Xuezhu Liu, Elo Harald Hansen

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

A sequential injection analysis system is described that incorporates a nylon tubular reactor containing immobilised glucose oxidase, allowing determination of D-glucose by means of subsequent luminol chemiluminescence detection of the hydrogen peroxide generated in the enzymatic reaction. The operating parameters were optimised by fractional factorial screening and response surface modelling. The linear range of D-glucose determination was 30-600 mu M, With a detection limit of 15 mu M using a photodiode detector. The sampling frequency was 54 h(-1). Lower LOD (0.5 mu M D-glucose) could be reached by using a PMT as the detector. Fermentation broth samples were determined and good recoveries were obtained.
Original languageEnglish
JournalAnalytica Chimica Acta
Volume326
Issue number1-3
Pages (from-to)1-12
ISSN0030-2670
DOIs
Publication statusPublished - 1996
EventFifth National Conference on Flow Injection Analysis - Qindao, China
Duration: 1 Jan 1996 → …

Conference

ConferenceFifth National Conference on Flow Injection Analysis
CityQindao, China
Period01/01/1996 → …

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