Sequential Injection Determination of D-Glucose by Chemiluminescence Using an Open Tubular Immobilised Enzyme Reactor

Xuezhu Liu; Elo Harald Hansen

doi:10.1016/0003-2670(96)00045-1

Sequential Injection Determination of D-Glucose by Chemiluminescence Using an Open Tubular Immobilised Enzyme Reactor

Xuezhu Liu, Elo Harald Hansen

Department of Chemistry

Research output: Contribution to journal › Journal article › Research › peer-review

Abstract

A sequential injection analysis system is described that incorporates a nylon tubular reactor containing immobilised glucose oxidase, allowing determination of D-glucose by means of subsequent luminol chemiluminescence detection of the hydrogen peroxide generated in the enzymatic reaction. The operating parameters were optimised by fractional factorial screening and response surface modelling. The linear range of D-glucose determination was 30-600 mu M, With a detection limit of 15 mu M using a photodiode detector. The sampling frequency was 54 h(-1). Lower LOD (0.5 mu M D-glucose) could be reached by using a PMT as the detector. Fermentation broth samples were determined and good recoveries were obtained.

Original language	English
Journal	Analytica Chimica Acta
Volume	326
Issue number	1-3
Pages (from-to)	1-12
ISSN	0030-2670
DOIs	https://doi.org/10.1016/0003-2670(96)00045-1
Publication status	Published - 1996
Event	Fifth National Conference on Flow Injection Analysis - Qindao, China Duration: 1 Jan 1996 → …

Conference

Conference	Fifth National Conference on Flow Injection Analysis
City	Qindao, China
Period	01/01/1996 → …

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title = "Sequential Injection Determination of D-Glucose by Chemiluminescence Using an Open Tubular Immobilised Enzyme Reactor",

abstract = "A sequential injection analysis system is described that incorporates a nylon tubular reactor containing immobilised glucose oxidase, allowing determination of D-glucose by means of subsequent luminol chemiluminescence detection of the hydrogen peroxide generated in the enzymatic reaction. The operating parameters were optimised by fractional factorial screening and response surface modelling. The linear range of D-glucose determination was 30-600 mu M, With a detection limit of 15 mu M using a photodiode detector. The sampling frequency was 54 h(-1). Lower LOD (0.5 mu M D-glucose) could be reached by using a PMT as the detector. Fermentation broth samples were determined and good recoveries were obtained. ",

author = "Xuezhu Liu and Hansen, {Elo Harald}",

year = "1996",

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volume = "326",

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T1 - Sequential Injection Determination of D-Glucose by Chemiluminescence Using an Open Tubular Immobilised Enzyme Reactor

AU - Liu, Xuezhu

AU - Hansen, Elo Harald

PY - 1996

Y1 - 1996

N2 - A sequential injection analysis system is described that incorporates a nylon tubular reactor containing immobilised glucose oxidase, allowing determination of D-glucose by means of subsequent luminol chemiluminescence detection of the hydrogen peroxide generated in the enzymatic reaction. The operating parameters were optimised by fractional factorial screening and response surface modelling. The linear range of D-glucose determination was 30-600 mu M, With a detection limit of 15 mu M using a photodiode detector. The sampling frequency was 54 h(-1). Lower LOD (0.5 mu M D-glucose) could be reached by using a PMT as the detector. Fermentation broth samples were determined and good recoveries were obtained.

AB - A sequential injection analysis system is described that incorporates a nylon tubular reactor containing immobilised glucose oxidase, allowing determination of D-glucose by means of subsequent luminol chemiluminescence detection of the hydrogen peroxide generated in the enzymatic reaction. The operating parameters were optimised by fractional factorial screening and response surface modelling. The linear range of D-glucose determination was 30-600 mu M, With a detection limit of 15 mu M using a photodiode detector. The sampling frequency was 54 h(-1). Lower LOD (0.5 mu M D-glucose) could be reached by using a PMT as the detector. Fermentation broth samples were determined and good recoveries were obtained.

U2 - 10.1016/0003-2670(96)00045-1

DO - 10.1016/0003-2670(96)00045-1

M3 - Journal article

SN - 0030-2670

VL - 326

SP - 1

EP - 12

JO - Analytica Chimica Acta

JF - Analytica Chimica Acta

IS - 1-3

T2 - Fifth National Conference on Flow Injection Analysis

Y2 - 1 January 1996

ER -

Sequential Injection Determination of D-Glucose by Chemiluminescence Using an Open Tubular Immobilised Enzyme Reactor

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