TY - JOUR
T1 - Sequence variation in the alpha-toxin encoding plc gene of Clostridium perfringens strains isolated from diseased and healthy chickens
AU - Abildgaard, L
AU - Engberg, RM
AU - Pedersen, Karl
AU - Schramm, A
AU - Højberg, O
PY - 2009
Y1 - 2009
N2 - The aim of the present study was to analyse the genetic diversity of the alpha-toxin encoding plc gene and the variation in a-toxin production of Clostridium perfringens type A strains isolated from presumably healthy chickens and chickens suffering from either necrotic enteritis (NE) or cholangio-hepatitis. The a-toxin encoding plc genes from 60 different pulsed-field gel electrophoresis (PFGE) types (strains) of C perfringens were sequenced and translated in silico to amino acid sequences and the a-toxin production was investigated in batch cultures of 45 of the strains using an enzyme-linked immunosorbent assay (ELISA) approach. Overall, the truncated amino acid sequences showed close similarity (> 98% at the amino acid level) to previously reported sequences from chicken-derived C. perfringens isolates. Variations were however observed in 23 out of 379 aa positions leading to the definition of 26 different a-toxin sequence types among the 60 strains. Moreover, a type II intron of 834 non-coding nucleotides was identified in the pic gene of three of the investigated strains. The in vitro alpha-toxin production investigated in 45 of the strains, including the three harbouring the intron, revealed no correlation between PFGE type, alpha-toxin sequence type, health status of the host chickens and level of a-toxin production. It is therefore concluded that neither pic gene type nor alpha-toxin production level seems to correlate to origin (healthy or diseased chicken) of the C perfringens strains. (C) 2008 Elsevier B.V. All rights reserved.
AB - The aim of the present study was to analyse the genetic diversity of the alpha-toxin encoding plc gene and the variation in a-toxin production of Clostridium perfringens type A strains isolated from presumably healthy chickens and chickens suffering from either necrotic enteritis (NE) or cholangio-hepatitis. The a-toxin encoding plc genes from 60 different pulsed-field gel electrophoresis (PFGE) types (strains) of C perfringens were sequenced and translated in silico to amino acid sequences and the a-toxin production was investigated in batch cultures of 45 of the strains using an enzyme-linked immunosorbent assay (ELISA) approach. Overall, the truncated amino acid sequences showed close similarity (> 98% at the amino acid level) to previously reported sequences from chicken-derived C. perfringens isolates. Variations were however observed in 23 out of 379 aa positions leading to the definition of 26 different a-toxin sequence types among the 60 strains. Moreover, a type II intron of 834 non-coding nucleotides was identified in the pic gene of three of the investigated strains. The in vitro alpha-toxin production investigated in 45 of the strains, including the three harbouring the intron, revealed no correlation between PFGE type, alpha-toxin sequence type, health status of the host chickens and level of a-toxin production. It is therefore concluded that neither pic gene type nor alpha-toxin production level seems to correlate to origin (healthy or diseased chicken) of the C perfringens strains. (C) 2008 Elsevier B.V. All rights reserved.
U2 - 10.1016/j.vetmic.2008.11.001
DO - 10.1016/j.vetmic.2008.11.001
M3 - Journal article
C2 - 19070974
SN - 0378-1135
VL - 36
SP - 293
EP - 299
JO - Veterinary Microbiology
JF - Veterinary Microbiology
IS - 3-4
ER -
