Sequence-selective recognition of DNA by strand displacement with a thymine-substituted polyamide

P.E. Nielsen, M. Egholm, R.H. Berg, O. Buchardt

    Research output: Contribution to journalJournal articleResearch

    Abstract

    A polyamide nucleic acid (PNA) was designed by detaching the deoxyribose phosphate backbone of DNA in a computer model and replacing it with an achiral polyamide backbone. On the basis of this model, oligomers consisting of thymine-linked aminoethylglycyl units were prepared. These oligomers recognize their complementary target in double-stranded DNA by strand displacement The displacement is made possible by the extraordinarily high stability of the PNA-DNA hybrids. The results show that the backbone of DNA can be replaced by a polyamide, with the resulting oligomer retaining base-specific hybridization.
    Original languageEnglish
    JournalScience
    Volume254
    Issue number5037
    Pages (from-to)1497-1500
    ISSN0036-8075
    DOIs
    Publication statusPublished - 1991

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