Abstract
Two Small cryptic plasmids designated pONE429 and pONE430 were isolated from a rumen bacterium, Selenomonas ruminantium S20. The complete sequence of pONE429 was 2100 bp and contained one open reading frame (ORF) of 201 amino acids. The sequence of pONE430 had 1527 bp and one ORF of 171 amino acids with the similarity of replication protein (Rep protein) of pOM1, pSN2, and pIM13 isolated from Butyrivibrio fibrisolvens, Staphylococcus aureus, and Bacillus subtilis, respectively. In these plasmids, the upstream nucleotide sequence of Rep protein had the conserved nucleotides which could be double-strand origin (DSO) of rolling circle replication (RCR) mechanism. The plasmids of pONE429, pONE430, pJJMI, pJDB21, and pS23 were isolated from S. ruminantium strains and had similar regions that were located within a
| Original language | English |
|---|---|
| Journal | Current Microbiology |
| Volume | 38 |
| Issue number | 2 |
| Pages (from-to) | 107-112 |
| Number of pages | 6 |
| ISSN | 0343-8651 |
| DOIs | |
| Publication status | Published - 1999 |
| Externally published | Yes |
Keywords
- Amino Acid Sequence
- Animals
- Base Sequence
- Blotting, Southern
- Molecular Sequence Data
- Open Reading Frames
- Plasmids
- Rumen
- Selenomonas
- helicase
- plasmid vector
- anaerobic bacterium
- article
- Bacillus subtilis
- molecular cloning
- nonhuman
- open reading frame
- priority journal
- Ruminococcus
- Selenomonas ruminantium
- sequence analysis
- Staphylococcus aureus
- Vibrio
- Bacteria (microorganisms)
- Butyrivibrio fibrisolvens
- Posibacteria
- MICROBIOLOGY
- ROLLING-CIRCLE PLASMIDS
- BUTYRIVIBRIO-FIBRISOLVENS
- STAPHYLOCOCCUS-AUREUS
- NUCLEOTIDE-SEQUENCE
- ESCHERICHIA-COLI
- RUMEN BACTERIUM
- REPLICATION
- BACTEROIDES
- VECTOR
- Anaerobic Gram-Negative Rods Eubacteria Bacteria Microorganisms (Bacteria, Eubacteria, Microorganisms) - Bacteroidaceae [06901] Selenomonas ruminantium strain-S20
- Artiodactyla Mammalia Vertebrata Chordata Animalia (Animals, Artiodactyls, Chordates, Mammals, Nonhuman Vertebrates, Nonhuman Mammals, Vertebrates) - Bovidae [85715] cow
- amino acids
- nucleotides
- replication proteins
- small cryptic plasmids isolation, sequence analysis, nucleotide sequence
- 00532, General biology - Miscellaneous
- 10010, Comparative biochemistry
- 10052, Biochemistry methods - Nucleic acids, purines and pyrimidines
- 10054, Biochemistry methods - Proteins, peptides and amino acids
- 10300, Replication, transcription, translation
- 10506, Biophysics - Molecular properties and macromolecules
- 13002, Metabolism - General metabolism and metabolic pathways
- 14001, Digestive system - General and methods
- 30500, Morphology and cytology of bacteria
- 31000, Physiology and biochemistry of bacteria
- 31500, Genetics of bacteria and viruses
- 36002, Medical and clinical microbiology - Bacteriology
- 38006, Veterinary science - Microbiology
- Biochemistry and Molecular Biophysics
- Molecular Genetics