Separation of cancer cells from white blood cells by pinched flow fractionation

Marie Pødenphant Jensen, Neil Ashley, Kamila Koprowska, Kalim U. Mir, Maksim Zalkovskij, Brian Bilenberg, Walter Bodmer, Anders Kristensen, Rodolphe Marie

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    Abstract

    In this paper, the microfluidic size-separation technique pinched flow fractionation (PFF) is used to separate cancer cells from white blood cells (WBCs). The cells are separated at efficiencies above 90% for both cell types. Circulating tumor cells (CTCs) are found in the blood of cancer patients and can form new tumors. CTCs are rare cells in blood, but they are important for the understanding of metastasis. There is therefore a high interest in developing a method for the enrichment of CTCs from blood samples, which also enables further analysis of the separated cells. The separation is challenged by the size overlap between cancer cells and the 106 times more abundant WBCs. The size overlap prevents high efficiency separation, however we demonstrate that cell deformability can be exploited in PFF devices to gain higher efficiencies than expected from the size distribution of the cells.
    Original languageEnglish
    JournalLab on a Chip
    Volume15
    Issue number24
    Pages (from-to)4598-4606
    ISSN1473-0197
    DOIs
    Publication statusPublished - 2015

    Bibliographical note

    This article is published Open Access as part of the RSC's Gold for Gold initiative, licensed under a Creative Commons Attribution 3.0 Unported Licence.

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