Separation and recovery of intracellular betacarotene using a process synthesis framework

Alexander M. Sabol, Maria-Ona Bertran, Jonathan P. Raftery, John M. Woodley, Rafiqul Gani, M. Nazmul Karima

Research output: Chapter in Book/Report/Conference proceedingArticle in proceedingsResearchpeer-review

Abstract

In this work, the process synthesis problem for the bio-manufacturing of high-value intracellular compounds is addressed using a systematic framework that allows for the user to input key process parameters from literature or experiments. The framework is based on a superstructure optimization approach and integrates various methods and tools, including a generic model and a database for data management (Bertran et al.,2017). We propose the following five steps: (1) problem formulation, (2) data collection and superstructure generation, (3) solution of the optimization problem, and (4) sensitivity analysis and (5) experimentation with informed design and then determination of the optimal process design. The framework is implemented in Super-O, software which guides the user through the formulation and solution of synthesis problems. This paper demonstrates the proposed framework though an illustrative case study on the production of beta-carotene from recombinant Saccharomyces cerevisiae(SM14) via continuous cultivation.
Original languageEnglish
Title of host publicationProceedings of the 27th European Symposium on Computer Aided Process Engineering (ESCAPE 27)
EditorsAntonio Espuña, Moisès Graells, Luis Puigjaner
Volume40
PublisherElsevier
Publication date2017
Edition1
Pages2851-2856
ISBN (Print)9780444639653
ISBN (Electronic)9780444639707
DOIs
Publication statusPublished - 2017
Event27th European Symposium on Computer Aided Process Engineering - Barcelona, Spain
Duration: 1 Oct 20175 Oct 2017
Conference number: 27
https://www.elsevier.com/books/27th-european-symposium-on-computer-aided-process-engineering/espuna/978-0-444-63965-3

Conference

Conference27th European Symposium on Computer Aided Process Engineering
Number27
CountrySpain
CityBarcelona
Period01/10/201705/10/2017
Internet address

Keywords

  • Process synthesis
  • Superstructure optimization
  • Downstream processing
  • Intracellular product
  • Bioprocess design
  • Bioseparations

Cite this

Sabol, A. M., Bertran, M-O., Raftery, J. P., Woodley, J. M., Gani, R., & Nazmul Karima, M. (2017). Separation and recovery of intracellular betacarotene using a process synthesis framework. In A. Espuña, M. Graells, & L. Puigjaner (Eds.), Proceedings of the 27th European Symposium on Computer Aided Process Engineering (ESCAPE 27) (1 ed., Vol. 40, pp. 2851-2856). Elsevier. https://doi.org/10.1016/B978-0-444-63965-3.50477-3