Separation and determination of alditols and sugars by high-pH anion-exchange chromatography with pulsed amperometric detection

Rikke Andersen, A. Sørensen

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

Carbohydrates such as alditols (polyols or sugar alcohols), monosaccharides and disaccharides are separated as anions by anion-exchange chromatography with a sodium hydroxide eluent, MA1 CarboPac column and pulsed amperometric detection. We report a high-pH anion-exchange chromatographic-pulsed amperometric detection (HPAEC-PAD) method that determines all the polyols used as food additives in food products and the most commonly found mono- and disaccharides on a routine basis. The linearity, repeatability, internal reproducibility and accuracy are described. The applicability of the method has been demonstrated by the analysis of 46 relevant samples and by participation twice in the Food Analysis Performance Assessment Scheme (FAPAS) testing programme for food additives. (C) 2000 Elsevier Science BN. All rights reserved.
Original languageEnglish
JournalJournal of Chromatography A
Volume897
Issue number1-2
Pages (from-to)195-204
ISSN0021-9673
Publication statusPublished - 2000

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