Separate and Simultaneous enzymatic hydrolysis and fermentation of wheat hemicellulose with recombinant xylose utilizing Saccharomyces cerevisiae

Lisbeth Olsson; H. R. Sørensen; B. P Dam; Kristian Bertel Rømer Mørkeberg Krogh; Anne Boye Strunge Meyer

doi:10.1385/ABAB:129:1:117

Separate and Simultaneous enzymatic hydrolysis and fermentation of wheat hemicellulose with recombinant xylose utilizing Saccharomyces cerevisiae

Lisbeth Olsson, H. R. Sørensen, B. P Dam, Kristian Bertel Rømer Mørkeberg Krogh, Anne Boye Strunge Meyer

Research output: Contribution to journal › Journal article › Research › peer-review

Abstract

Fermentations with three different xylose-utilizing recombinant Saccharomyces cerevisiae strains (F12, CR4, and CB4) were performed using two different wheat hemicellulose substrates, unfermented starch free fibers, and an industrial ethanol fermentation residue, vinasse. With CR4 and F12, the maximum ethanol concentrations obtained were 4.3 and 4 g/L, respectively, but F12 converted xylose 15% faster than CR4 during the first 24 h. The comparison of separate hydrolysis and fermentation (SHF) and simultaneous saccharification and fermentation (SSF) with F12 showed that the highest, maximum ethanol concentrations were obtained with SSF. In general, the volumetric ethanol productivity was initially, highest in the SHF, but the overall volumetric ethanol productivity ended up being maximal in the SSF, at 0.013 and 0.010 g/Lh, with starch free fibers and vinasse, respectively.

Original language	English
Journal	Applied Biochemistry and Biotechnology
Volume	129
Issue number	1-3
Pages (from-to)	117-129
ISSN	0273-2289
DOIs	https://doi.org/10.1385/ABAB:129:1:117
Publication status	Published - 2006

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title = "Separate and Simultaneous enzymatic hydrolysis and fermentation of wheat hemicellulose with recombinant xylose utilizing Saccharomyces cerevisiae",

abstract = "Fermentations with three different xylose-utilizing recombinant Saccharomyces cerevisiae strains (F12, CR4, and CB4) were performed using two different wheat hemicellulose substrates, unfermented starch free fibers, and an industrial ethanol fermentation residue, vinasse. With CR4 and F12, the maximum ethanol concentrations obtained were 4.3 and 4 g/L, respectively, but F12 converted xylose 15% faster than CR4 during the first 24 h. The comparison of separate hydrolysis and fermentation (SHF) and simultaneous saccharification and fermentation (SSF) with F12 showed that the highest, maximum ethanol concentrations were obtained with SSF. In general, the volumetric ethanol productivity was initially, highest in the SHF, but the overall volumetric ethanol productivity ended up being maximal in the SSF, at 0.013 and 0.010 g/Lh, with starch free fibers and vinasse, respectively. ",

author = "Lisbeth Olsson and S{\o}rensen, {H. R.} and Dam, {B. P} and Krogh, {Kristian Bertel R{\o}mer M{\o}rkeberg} and Meyer, {Anne Boye Strunge}",

year = "2006",

doi = "10.1385/ABAB:129:1:117",

language = "English",

volume = "129",

pages = "117--129",

journal = "Applied Biochemistry and Biotechnology",

issn = "0273-2289",

publisher = "Humana Press",

number = "1-3",

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Separate and Simultaneous enzymatic hydrolysis and fermentation of wheat hemicellulose with recombinant xylose utilizing Saccharomyces cerevisiae. / Olsson, Lisbeth; Sørensen, H. R.; Dam, B. P; Krogh, Kristian Bertel Rømer Mørkeberg; Meyer, Anne Boye Strunge.

In: Applied Biochemistry and Biotechnology, Vol. 129, No. 1-3, 2006, p. 117-129.

Research output: Contribution to journal › Journal article › Research › peer-review

TY - JOUR

T1 - Separate and Simultaneous enzymatic hydrolysis and fermentation of wheat hemicellulose with recombinant xylose utilizing Saccharomyces cerevisiae

AU - Olsson, Lisbeth

AU - Sørensen, H. R.

AU - Dam, B. P

AU - Krogh, Kristian Bertel Rømer Mørkeberg

AU - Meyer, Anne Boye Strunge

PY - 2006

Y1 - 2006

N2 - Fermentations with three different xylose-utilizing recombinant Saccharomyces cerevisiae strains (F12, CR4, and CB4) were performed using two different wheat hemicellulose substrates, unfermented starch free fibers, and an industrial ethanol fermentation residue, vinasse. With CR4 and F12, the maximum ethanol concentrations obtained were 4.3 and 4 g/L, respectively, but F12 converted xylose 15% faster than CR4 during the first 24 h. The comparison of separate hydrolysis and fermentation (SHF) and simultaneous saccharification and fermentation (SSF) with F12 showed that the highest, maximum ethanol concentrations were obtained with SSF. In general, the volumetric ethanol productivity was initially, highest in the SHF, but the overall volumetric ethanol productivity ended up being maximal in the SSF, at 0.013 and 0.010 g/Lh, with starch free fibers and vinasse, respectively.

AB - Fermentations with three different xylose-utilizing recombinant Saccharomyces cerevisiae strains (F12, CR4, and CB4) were performed using two different wheat hemicellulose substrates, unfermented starch free fibers, and an industrial ethanol fermentation residue, vinasse. With CR4 and F12, the maximum ethanol concentrations obtained were 4.3 and 4 g/L, respectively, but F12 converted xylose 15% faster than CR4 during the first 24 h. The comparison of separate hydrolysis and fermentation (SHF) and simultaneous saccharification and fermentation (SSF) with F12 showed that the highest, maximum ethanol concentrations were obtained with SSF. In general, the volumetric ethanol productivity was initially, highest in the SHF, but the overall volumetric ethanol productivity ended up being maximal in the SSF, at 0.013 and 0.010 g/Lh, with starch free fibers and vinasse, respectively.

U2 - 10.1385/ABAB:129:1:117

DO - 10.1385/ABAB:129:1:117

M3 - Journal article

VL - 129

SP - 117

EP - 129

JO - Applied Biochemistry and Biotechnology

JF - Applied Biochemistry and Biotechnology

SN - 0273-2289

IS - 1-3

ER -

Separate and Simultaneous enzymatic hydrolysis and fermentation of wheat hemicellulose with recombinant xylose utilizing Saccharomyces cerevisiae

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