Separate and Simultaneous enzymatic hydrolysis and fermentation of wheat hemicellulose with recombinant xylose utilizing Saccharomyces cerevisiae

Lisbeth Olsson, H. R. Sørensen, B. P Dam, Kristian Bertel Rømer Mørkeberg Krogh, Anne Boye Strunge Meyer

    Research output: Contribution to journalJournal articleResearchpeer-review

    Abstract

    Fermentations with three different xylose-utilizing recombinant Saccharomyces cerevisiae strains (F12, CR4, and CB4) were performed using two different wheat hemicellulose substrates, unfermented starch free fibers, and an industrial ethanol fermentation residue, vinasse. With CR4 and F12, the maximum ethanol concentrations obtained were 4.3 and 4 g/L, respectively, but F12 converted xylose 15% faster than CR4 during the first 24 h. The comparison of separate hydrolysis and fermentation (SHF) and simultaneous saccharification and fermentation (SSF) with F12 showed that the highest, maximum ethanol concentrations were obtained with SSF. In general, the volumetric ethanol productivity was initially, highest in the SHF, but the overall volumetric ethanol productivity ended up being maximal in the SSF, at 0.013 and 0.010 g/Lh, with starch free fibers and vinasse, respectively.
    Original languageEnglish
    JournalApplied Biochemistry and Biotechnology
    Volume129
    Issue number1-3
    Pages (from-to)117-129
    ISSN0273-2289
    DOIs
    Publication statusPublished - 2006

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