Sensor Systems with Magnetic and Optomagnetic Readout of Rolling Circle Amplification Products

Mikkel Fougt Hansen, Marco Donolato, Jeppe Fock, Mattias Strömberg, Maria Strømme, Peter Svedlindh

    Research output: Chapter in Book/Report/Conference proceedingBook chapterResearchpeer-review

    Abstract

    We are developing robust biosensors for homogeneous detection of rolling cirle amplification (RCA) products with magnetic and/or optomagnetic readouts based on surface-functionalized magnetic nanoparticles. Binding of RCA amplicons to nanoparticles modifies their ability to rotate in response to an applied oscillating magnetic field. As a result, magnetic or optical measurements of these changes in the rotational response of nanoparticles vs. frequency of the magnetic field can be used to quantitate the number of amplicons, and, hence, the concentration of target nucleic acid analytes. After describing the basic principles of this approach, we present the current status of the development of compact and portable sensing devices used to measure the dynamic response of magnetic particle suspensions. Then, we give examples and results of different RCA detection strategies designed by us, and we also outline future directions for this innovative diagnostic approach.
    Original languageEnglish
    Title of host publicationRolling Circle Amplification (RCA) : Toward New Clinical Diagnostics and Therapeutics
    EditorsVadim V. Demidov
    PublisherSpringer
    Publication date2016
    Edition1
    Pages123-128
    ISBN (Print)978-3-319-42224-4
    ISBN (Electronic)978-3-319-42226-8
    Publication statusPublished - 2016

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