Sensor combination and chemometric variable selection for online monitoring of Streptomyces coelicolor fed-batch cultivations

Peter Ödman, C.L. Johansen, L. Olsson, Krist Gernaey, Anna Eliasson Lantz

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

Fed-batch cultivations of Streptomyces coelicolor, producing the antibiotic actinorhodin, were monitored online by multiwavelength fluorescence spectroscopy and off-gas analysis. Partial least squares (PLS), locally weighted regression, and multilinear PLS (N-PLS) models were built for prediction of biomass and substrate (casamino acids) concentrations, respectively. The effect of combination of fluorescence and gas analyzer data as well as of different variable selection methods was investigated. Improved prediction models were obtained by combination of data from the two sensors and by variable selection using a genetic algorithm, interval PLS, and the principal variables method, respectively. A stepwise variable elimination method was applied to the three-way fluorescence data, resulting in simpler and more accurate N-PLS models. The prediction models were validated using leave-one-batch-out cross-validation, and the best models had root mean square error of cross-validation values of 1.02 g l(-1) biomass and 0.8 g l(-1) total amino acids, respectively. The fluorescence data were also explored by parallel factor analysis. The analysis revealed four spectral profiles present in the fluorescence data, three of which were identified as pyridoxine, NAD(P)H, and flavin nucleotides, respectively.
Original languageEnglish
JournalApplied Microbiology and Biotechnology
Volume86
Issue number6
Pages (from-to)1745-1759
ISSN0175-7598
DOIs
Publication statusPublished - 2010

Keywords

  • Genetic algorithm
  • PLS
  • Multiwavelength fluorescence
  • Bioprocess monitoring
  • LWR
  • iPLS
  • Principal variables

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