Sensitive Blu-ray detection of clustered rolling circle products for molecular diagnostics

A. Ahlford; Marco Donolato; A. Mezger; Paula Soares Martins Antunes; Frederik Westergaard Østerberg; Robert Burger; Filippo Bosco; Anja Boisen; Mikkel Fougt Hansen; M. Nilsson

Sensitive Blu-ray detection of clustered rolling circle products for molecular diagnostics

A. Ahlford, Marco Donolato, A. Mezger, Paula Soares Martins Antunes, Frederik Westergaard Østerberg, Robert Burger, Filippo Bosco, Anja Boisen, Mikkel Fougt Hansen, M. Nilsson

Stockholm University

Research output: Chapter in Book/Report/Conference proceeding › Article in proceedings › Research › peer-review

Abstract

In this paper we present a method for low cost and rapid sensing of nucleic acids (NA) for infectious diagnostics, where isothermal rolling circle amplification (RCA) products, specifically generated by the presence of the human pathogen Pseudomonas aeruginosa (PA), are bound to magnetic nanoparticles (MNP). Samples are injected into a microfluidic disc chip and analyzed on a novel magneto-optical platform. The sensing approach is based on the clustering pattern of MNP in a magnetic field and measures the effect of cluster formation on transmitted light using a standard Blu-Ray pickup head. The concentration of DNA target is correlated with an increase of clustered particles.

Original language	English
Title of host publication	Proceedings of the 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences
Publisher	Chemical and Biological Microsystems Society
Publication date	2014
Pages	24-26
ISBN (Print)	978-0-9798064-7-6
Publication status	Published - 2014
Event	18th International Conference on Miniaturized Systems for Chemistry and Life Sciences - San Antonio, United States Duration: 26 Oct 2014 → 30 Oct 2014 Conference number: 18 https://microtasconferences.org/microtas2014/

Conference

Conference	18th International Conference on Miniaturized Systems for Chemistry and Life Sciences
Number	18
Country/Territory	United States
City	San Antonio
Period	26/10/2014 → 30/10/2014
Internet address	https://microtasconferences.org/microtas2014/

Keywords

Molecular diagnostics
Magnetic biosensing
Optical scattering
DNA amplifications
Lab- on-a-chip

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Ahlford, A., Donolato, M., Mezger, A., Antunes, P. S. M., Østerberg, F. W., Burger, R., Bosco, F., Boisen, A., Hansen, M. F., & Nilsson, M. (2014). Sensitive Blu-ray detection of clustered rolling circle products for molecular diagnostics. In Proceedings of the 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences (pp. 24-26). Chemical and Biological Microsystems Society.

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abstract = "In this paper we present a method for low cost and rapid sensing of nucleic acids (NA) for infectious diagnostics, where isothermal rolling circle amplification (RCA) products, specifically generated by the presence of the human pathogen Pseudomonas aeruginosa (PA), are bound to magnetic nanoparticles (MNP). Samples are injected into a microfluidic disc chip and analyzed on a novel magneto-optical platform. The sensing approach is based on the clustering pattern of MNP in a magnetic field and measures the effect of cluster formation on transmitted light using a standard Blu-Ray pickup head. The concentration of DNA target is correlated with an increase of clustered particles.",

keywords = "Molecular diagnostics, Magnetic biosensing, Optical scattering, DNA amplifications, Lab- on-a-chip",

author = "A. Ahlford and Marco Donolato and A. Mezger and Antunes, {Paula Soares Martins} and {\O}sterberg, {Frederik Westergaard} and Robert Burger and Filippo Bosco and Anja Boisen and Hansen, {Mikkel Fougt} and M. Nilsson",

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pages = "24--26",

booktitle = "Proceedings of the 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences",

publisher = "Chemical and Biological Microsystems Society",

note = "18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014 ; Conference date: 26-10-2014 Through 30-10-2014",

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Ahlford, A, Donolato, M, Mezger, A, Antunes, PSM, Østerberg, FW, Burger, R, Bosco, F, Boisen, A, Hansen, MF & Nilsson, M 2014, Sensitive Blu-ray detection of clustered rolling circle products for molecular diagnostics. in Proceedings of the 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Chemical and Biological Microsystems Society, pp. 24-26, 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, San Antonio, Texas, United States, 26/10/2014.

Sensitive Blu-ray detection of clustered rolling circle products for molecular diagnostics. / Ahlford, A.; Donolato, Marco; Mezger, A. et al.
Proceedings of the 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences. Chemical and Biological Microsystems Society, 2014. p. 24-26.

Research output: Chapter in Book/Report/Conference proceeding › Article in proceedings › Research › peer-review

TY - GEN

T1 - Sensitive Blu-ray detection of clustered rolling circle products for molecular diagnostics

AU - Ahlford, A.

AU - Donolato, Marco

AU - Mezger, A.

AU - Antunes, Paula Soares Martins

AU - Østerberg, Frederik Westergaard

AU - Burger, Robert

AU - Bosco, Filippo

AU - Boisen, Anja

AU - Hansen, Mikkel Fougt

AU - Nilsson, M.

N1 - Conference code: 18

PY - 2014

Y1 - 2014

N2 - In this paper we present a method for low cost and rapid sensing of nucleic acids (NA) for infectious diagnostics, where isothermal rolling circle amplification (RCA) products, specifically generated by the presence of the human pathogen Pseudomonas aeruginosa (PA), are bound to magnetic nanoparticles (MNP). Samples are injected into a microfluidic disc chip and analyzed on a novel magneto-optical platform. The sensing approach is based on the clustering pattern of MNP in a magnetic field and measures the effect of cluster formation on transmitted light using a standard Blu-Ray pickup head. The concentration of DNA target is correlated with an increase of clustered particles.

AB - In this paper we present a method for low cost and rapid sensing of nucleic acids (NA) for infectious diagnostics, where isothermal rolling circle amplification (RCA) products, specifically generated by the presence of the human pathogen Pseudomonas aeruginosa (PA), are bound to magnetic nanoparticles (MNP). Samples are injected into a microfluidic disc chip and analyzed on a novel magneto-optical platform. The sensing approach is based on the clustering pattern of MNP in a magnetic field and measures the effect of cluster formation on transmitted light using a standard Blu-Ray pickup head. The concentration of DNA target is correlated with an increase of clustered particles.

KW - Molecular diagnostics

KW - Magnetic biosensing

KW - Optical scattering

KW - DNA amplifications

KW - Lab- on-a-chip

M3 - Article in proceedings

SN - 978-0-9798064-7-6

SP - 24

EP - 26

BT - Proceedings of the 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences

PB - Chemical and Biological Microsystems Society

T2 - 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences

Y2 - 26 October 2014 through 30 October 2014

ER -

Sensitive Blu-ray detection of clustered rolling circle products for molecular diagnostics

Abstract

Conference

Keywords

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