Sensitive Blu-ray detection of clustered rolling circle products for molecular diagnostics

A. Ahlford, Marco Donolato, A. Mezger, Paula Soares Martins Antunes, Frederik Westergaard Østerberg, Robert Burger, Filippo Bosco, Anja Boisen, Mikkel Fougt Hansen, M. Nilsson

    Research output: Chapter in Book/Report/Conference proceedingArticle in proceedingsResearchpeer-review

    Abstract

    In this paper we present a method for low cost and rapid sensing of nucleic acids (NA) for infectious diagnostics, where isothermal rolling circle amplification (RCA) products, specifically generated by the presence of the human pathogen Pseudomonas aeruginosa (PA), are bound to magnetic nanoparticles (MNP). Samples are injected into a microfluidic disc chip and analyzed on a novel magneto-optical platform. The sensing approach is based on the clustering pattern of MNP in a magnetic field and measures the effect of cluster formation on transmitted light using a standard Blu-Ray pickup head. The concentration of DNA target is correlated with an increase of clustered particles.
    Original languageEnglish
    Title of host publicationProceedings of the 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences
    PublisherChemical and Biological Microsystems Society
    Publication date2014
    Pages24-26
    ISBN (Print)978-0-9798064-7-6
    Publication statusPublished - 2014
    Event18th International Conference on Miniaturized Systems for Chemistry and Life Sciences - Texas, San Antonio, United States
    Duration: 26 Oct 201430 Oct 2014
    Conference number: 18

    Conference

    Conference18th International Conference on Miniaturized Systems for Chemistry and Life Sciences
    Number18
    LocationTexas
    CountryUnited States
    CitySan Antonio
    Period26/10/201430/10/2014

    Keywords

    • Molecular diagnostics
    • Magnetic biosensing
    • Optical scattering
    • DNA amplifications
    • Lab- on-a-chip

    Cite this

    Ahlford, A., Donolato, M., Mezger, A., Antunes, P. S. M., Østerberg, F. W., Burger, R., Bosco, F., Boisen, A., Hansen, M. F., & Nilsson, M. (2014). Sensitive Blu-ray detection of clustered rolling circle products for molecular diagnostics. In Proceedings of the 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences (pp. 24-26). Chemical and Biological Microsystems Society.