Self-assembled nanofibrils from RGD-functionalized cellulose nanocrystals to improve the performance of PEI/DNA polyplexes

Sry D. Hujaya, Aki Manninen, Kirsten Inga Kling, Jakob Birkedal Wagner, Seppo J. Vainio, Henrikki Liimatainen*

*Corresponding author for this work

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

Cellulose nanocrystals (CNCs) are promising bio-derived nanomaterials for the bottom-up fabrication of biomedical constructs. In this report, dicarboxylic acid-functionalized CNC (DCC) was functionalized with arginylglycylaspartic acid (RGD) tripeptide as a motif for improved cell adhesion and targeting. The product (DCC-RGD) self-assembled into a more elongated nanofibrillar structure through lateral and end-to-end association. When added into poly(ethylene imine) (PEI)/pDNA polyplex solution, nanocelluloses interacted electrostatically with positively charged polyplexes without affecting their integrity. The constructs were tested for their potentials as non-viral transfection reagents. Cell viability and transfection efficiency of fibroblast NIH3T3 cells were monitored as a function of CNC concentration where, in general, viability increased as the CNC concentration increased, and transfection efficiency could be optimized. Using wild-type MDCK and αV-knockout MDCK cells, the construct was able to provide targeted uptake of polyplexes. The findings have potential applications, for example, cell-selective in vitro or ex vivo transfection of autologous mesenchymal stem cells for cell therapy, or bottom-up design of future innovative biomaterials.
Original languageEnglish
JournalJournal of Colloid and Interface Science
Volume553
Pages (from-to)71-82
ISSN0021-9797
DOIs
Publication statusPublished - 2019

Keywords

  • Cellulose nanocrystals
  • Gene therapy
  • Poly(ethylene imine)
  • RGD
  • Targeting
  • Transfection

Cite this

Hujaya, Sry D. ; Manninen, Aki ; Kling, Kirsten Inga ; Wagner, Jakob Birkedal ; Vainio, Seppo J. ; Liimatainen, Henrikki. / Self-assembled nanofibrils from RGD-functionalized cellulose nanocrystals to improve the performance of PEI/DNA polyplexes. In: Journal of Colloid and Interface Science. 2019 ; Vol. 553. pp. 71-82.
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abstract = "Cellulose nanocrystals (CNCs) are promising bio-derived nanomaterials for the bottom-up fabrication of biomedical constructs. In this report, dicarboxylic acid-functionalized CNC (DCC) was functionalized with arginylglycylaspartic acid (RGD) tripeptide as a motif for improved cell adhesion and targeting. The product (DCC-RGD) self-assembled into a more elongated nanofibrillar structure through lateral and end-to-end association. When added into poly(ethylene imine) (PEI)/pDNA polyplex solution, nanocelluloses interacted electrostatically with positively charged polyplexes without affecting their integrity. The constructs were tested for their potentials as non-viral transfection reagents. Cell viability and transfection efficiency of fibroblast NIH3T3 cells were monitored as a function of CNC concentration where, in general, viability increased as the CNC concentration increased, and transfection efficiency could be optimized. Using wild-type MDCK and αV-knockout MDCK cells, the construct was able to provide targeted uptake of polyplexes. The findings have potential applications, for example, cell-selective in vitro or ex vivo transfection of autologous mesenchymal stem cells for cell therapy, or bottom-up design of future innovative biomaterials.",
keywords = "Cellulose nanocrystals, Gene therapy, Poly(ethylene imine), RGD, Targeting, Transfection",
author = "Hujaya, {Sry D.} and Aki Manninen and Kling, {Kirsten Inga} and Wagner, {Jakob Birkedal} and Vainio, {Seppo J.} and Henrikki Liimatainen",
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Self-assembled nanofibrils from RGD-functionalized cellulose nanocrystals to improve the performance of PEI/DNA polyplexes. / Hujaya, Sry D.; Manninen, Aki; Kling, Kirsten Inga; Wagner, Jakob Birkedal; Vainio, Seppo J.; Liimatainen, Henrikki.

In: Journal of Colloid and Interface Science, Vol. 553, 2019, p. 71-82.

Research output: Contribution to journalJournal articleResearchpeer-review

TY - JOUR

T1 - Self-assembled nanofibrils from RGD-functionalized cellulose nanocrystals to improve the performance of PEI/DNA polyplexes

AU - Hujaya, Sry D.

AU - Manninen, Aki

AU - Kling, Kirsten Inga

AU - Wagner, Jakob Birkedal

AU - Vainio, Seppo J.

AU - Liimatainen, Henrikki

PY - 2019

Y1 - 2019

N2 - Cellulose nanocrystals (CNCs) are promising bio-derived nanomaterials for the bottom-up fabrication of biomedical constructs. In this report, dicarboxylic acid-functionalized CNC (DCC) was functionalized with arginylglycylaspartic acid (RGD) tripeptide as a motif for improved cell adhesion and targeting. The product (DCC-RGD) self-assembled into a more elongated nanofibrillar structure through lateral and end-to-end association. When added into poly(ethylene imine) (PEI)/pDNA polyplex solution, nanocelluloses interacted electrostatically with positively charged polyplexes without affecting their integrity. The constructs were tested for their potentials as non-viral transfection reagents. Cell viability and transfection efficiency of fibroblast NIH3T3 cells were monitored as a function of CNC concentration where, in general, viability increased as the CNC concentration increased, and transfection efficiency could be optimized. Using wild-type MDCK and αV-knockout MDCK cells, the construct was able to provide targeted uptake of polyplexes. The findings have potential applications, for example, cell-selective in vitro or ex vivo transfection of autologous mesenchymal stem cells for cell therapy, or bottom-up design of future innovative biomaterials.

AB - Cellulose nanocrystals (CNCs) are promising bio-derived nanomaterials for the bottom-up fabrication of biomedical constructs. In this report, dicarboxylic acid-functionalized CNC (DCC) was functionalized with arginylglycylaspartic acid (RGD) tripeptide as a motif for improved cell adhesion and targeting. The product (DCC-RGD) self-assembled into a more elongated nanofibrillar structure through lateral and end-to-end association. When added into poly(ethylene imine) (PEI)/pDNA polyplex solution, nanocelluloses interacted electrostatically with positively charged polyplexes without affecting their integrity. The constructs were tested for their potentials as non-viral transfection reagents. Cell viability and transfection efficiency of fibroblast NIH3T3 cells were monitored as a function of CNC concentration where, in general, viability increased as the CNC concentration increased, and transfection efficiency could be optimized. Using wild-type MDCK and αV-knockout MDCK cells, the construct was able to provide targeted uptake of polyplexes. The findings have potential applications, for example, cell-selective in vitro or ex vivo transfection of autologous mesenchymal stem cells for cell therapy, or bottom-up design of future innovative biomaterials.

KW - Cellulose nanocrystals

KW - Gene therapy

KW - Poly(ethylene imine)

KW - RGD

KW - Targeting

KW - Transfection

U2 - 10.1016/j.jcis.2019.06.001

DO - 10.1016/j.jcis.2019.06.001

M3 - Journal article

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SN - 0021-9797

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