Selective Serial Multi-Antibody Biosensing with TOPAS Microstructured Polymer Optical Fibers

Grigoriy Andreev Emiliyanov; Poul E. Høiby; Lars H. Pedersen; Ole Bang

doi:10.3390/s130303242

Selective Serial Multi-Antibody Biosensing with TOPAS Microstructured Polymer Optical Fibers

Grigoriy Andreev Emiliyanov, Poul E. Høiby, Lars H. Pedersen, Ole Bang

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Abstract

We have developed a fluorescence-based fiber-optical biosensor, which can selectively detect different antibodies in serial at preselected positions inside a single piece of fiber. The fiber is a microstructured polymer optical fiber fabricated from TOPAS cyclic olefin copolymer, which allows for UV activation of localized sensor layers inside the holes of the fiber. Serial fluorescence-based selective sensing of Cy3-labelled α-streptavidin and Cy5-labelled α-CRP antibodies is demonstrated.

Original language	English
Journal	Sensors
Volume	13
Issue number	3
Pages (from-to)	3242-3251
ISSN	1424-8220
DOIs	https://doi.org/10.3390/s130303242
Publication status	Published - 2013

Bibliographical note

© 2013 by the authors; licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).

Keywords

Microstructured polymer optical fiber
Fluorescence
Antibodies

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Sensors 13 03242Final published version, 449 KB

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AU - Bang, Ole

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N2 - We have developed a fluorescence-based fiber-optical biosensor, which can selectively detect different antibodies in serial at preselected positions inside a single piece of fiber. The fiber is a microstructured polymer optical fiber fabricated from TOPAS cyclic olefin copolymer, which allows for UV activation of localized sensor layers inside the holes of the fiber. Serial fluorescence-based selective sensing of Cy3-labelled α-streptavidin and Cy5-labelled α-CRP antibodies is demonstrated.

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KW - Antibodies

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Selective Serial Multi-Antibody Biosensing with TOPAS Microstructured Polymer Optical Fibers

Abstract

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Keywords

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