Selective label-free electrochemical impedance measurement of glycated haemoglobin on 3-aminophenylboronic acid-modified eggshell membranes

Yuwadee Boonyasit, Arto Heiskanen, Orawan Chailapakul, Wanida Laiwattanapaisal

    Research output: Contribution to journalJournal articleResearchpeer-review

    Abstract

    We propose a novel alternative approach to long-term glycaemic monitoring using eggshell membranes (ESMs) as a new immobilising platform for the selective label-free electrochemical sensing of glycated haemoglobin (HbA1c), a vital clinical index of the glycaemic status in diabetic individuals. Due to the unique features of a novel 3-aminophenylboronic acid-modified ESM, selective binding was obtained via cis–diol interactions. This newly developed device provides clinical applicability as an affinity membrane-based biosensor for the identification of HbA1c over a clinically relevant range (2.3 - 14 %) with a detection limit of 0.19 %. The proposed membrane-based biosensor also exhibited good reproducibility. When analysing normal and abnormal HbA1c levels, the within-run coefficients of variation were 1.68 and 1.83 %, respectively. The run-to-run coefficients of variation were 1.97 and 2.02 %, respectively. These results demonstrated that this method achieved the precise and selective measurement of HbA1c. Compared with a commercial HbA1c kit, the results demonstrated excellent agreement between the techniques (n=15), demonstrating the clinical applicability of this sensor for monitoring glycaemic control. Thus, this low-cost sensing platform using the proposed membrane-based biosensor is ideal for point-of-care diagnostics.
    Original languageEnglish
    JournalAnalytical and Bioanalytical Chemistry
    Volume407
    Issue number18
    Pages (from-to)5287-5297
    Number of pages11
    ISSN1618-2642
    DOIs
    Publication statusPublished - 2015

    Keywords

    • HASH (0x533daa8)
    • Glycated haemoglobin (HbA1c)
    • Diabetes mellitus
    • 3-aminophenyl boronic acid
    • Eggshell membrane
    • Membrane-based biosensor
    • Selective label-free electrochemical detection

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