Selective flocculation and precipitation for the improvement of virus-like particle recovery from yeast homogenate

S. Tsoka, O. C. Ciniawskyj, Owen R. T. Thomas, N. J. Titchener-Hooker, M. Hoare

    Research output: Contribution to journalJournal articleResearchpeer-review

    Abstract

    The purification of an intracellular product from a complex mixture of contaminants after cell disruption is a common problem in processes downstream of fermentation systems. This is particularly challenging for the recovery of particulate (80 nm in diameter) multimeric protein products, named virus-like particles (VLPs), from cell debris and other intracellular components. Selective flocculation for debris removal followed by selective precipitation of the target protein can be used as a preclarification step to aid purification. In this paper, selective borax flocculation of cell debris in yeast homogenate, followed by selective poly(ethylene glycol) precipitation of VLPs are defined with a view to demonstrating their potential in aiding the initial clarification stages of the purification sequence. The translation from laboratory scale to pilot scale operation is addressed, demonstrating the challenge of scale-up of solid-liquid separation stages for biological particle processing.
    Original languageEnglish
    JournalBiotechnology Progress
    Volume16
    Issue number4
    Pages (from-to)661-667
    ISSN8756-7938
    DOIs
    Publication statusPublished - 2000

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