We demonstrate selective detection of fluorophore labeled antibodies from minute samples probed by a sensor layer of complementary biomolecules immobilized inside the air holes of microstructured Polymer Optical Fiber (mPOF). The fiber core is defined by a ring of 6 air holes and a simple procedure was applied to selectively capture either α-streptavidin or α-CRP antibodies inside these air holes. A sensitive and easy-to-use fluorescence method was used for the optical detection. Our results show that mPOF based biosensors can provide reliable and selective antibody detection in ultra small sample volumes.
|Publication status||Published - 2005|
Jensen, J. B. D., Hoiby, P. E., Emiliyanov, G. A., Bang, O., Pedersen, L. H., & Bjarklev, A. O. (2005). Selective detection of antibodies in microstructured polymer optical fibers. Optics Express, 13(15), 5883-5889. http://www.opticsexpress.org/abstract.cfm?URI=OPEX-13-15-5883