Abstract
We demonstrate selective detection of fluorophore labeled antibodies from minute samples probed by a sensor layer of complementary biomolecules immobilized inside the air holes of microstructured Polymer Optical Fiber (mPOF). The fiber core is defined by a ring of 6 air holes and a simple procedure was applied to selectively capture either α-streptavidin or α-CRP antibodies inside these air holes. A sensitive and easy-to-use fluorescence method was used for the optical detection. Our results show that mPOF based biosensors can provide reliable and selective antibody detection in ultra small sample volumes.
Original language | English |
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Journal | Optics Express |
Volume | 13 |
Issue number | 15 |
Pages (from-to) | 5883-5889 |
ISSN | 1094-4087 |
Publication status | Published - 2005 |