Selective detection of antibodies in microstructured polymer optical fibers

Jesper Bo Damm Jensen, P.E. Hoiby, Grigoriy Andreev Emiliyanov, Ole Bang, L.H. Pedersen, Anders Overgaard Bjarklev

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

We demonstrate selective detection of fluorophore labeled antibodies from minute samples probed by a sensor layer of complementary biomolecules immobilized inside the air holes of microstructured Polymer Optical Fiber (mPOF). The fiber core is defined by a ring of 6 air holes and a simple procedure was applied to selectively capture either α-streptavidin or α-CRP antibodies inside these air holes. A sensitive and easy-to-use fluorescence method was used for the optical detection. Our results show that mPOF based biosensors can provide reliable and selective antibody detection in ultra small sample volumes.
Original languageEnglish
JournalOptics Express
Volume13
Issue number15
Pages (from-to)5883-5889
ISSN1094-4087
Publication statusPublished - 2005

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