Selection of hyperadherent mutants in Pseudomonas putida biofilms

Fatima Yousef-Coronado, María Isabel Soriano, Liang Yang, Søren Molin, Manuel Espinosa-Urgel

    Research output: Contribution to journalJournal articleResearchpeer-review

    Abstract

    A number of genetic determinants required for bacterial colonization of solid surfaces and biofilm formation have been identified in different micro-organisms. There are fewer accounts of mutations that favour the transition to a sessile mode of life. Here we report the isolation of random transposon Pseudomonas putida KT2440 mutants showing increased biofilm formation, and the detailed characterization of one of them. This mutant exhibits a complex phenotype, including altered colony morphology, increased production of extracellular polymeric substances and enhanced swarming motility, along with the formation of denser and more complex biofilms than the parental strain. Sequence analysis revealed that the pleiotropic phenotype exhibited by the mutant resulted from the accumulation of two mutations: a transposon insertion, which disrupted a predicted outer membrane lipoprotein, and a point mutation in lapG, a gene involved in the turnover of the large adhesin LapA. The contribution of each alteration to the phenotype and the possibility that prolonged sessile growth results in the selection of hyperadherent mutants are discussed.
    Original languageEnglish
    JournalMicrobiology
    Volume157
    Issue numberPt 8
    Pages (from-to)2257-2265
    ISSN1350-0872
    DOIs
    Publication statusPublished - 2011

    Fingerprint Dive into the research topics of 'Selection of hyperadherent mutants in Pseudomonas putida biofilms'. Together they form a unique fingerprint.

    Cite this