Selection of Bacillus species for targeted in situ release of prebiotic galacto-rhamnogalacturonan from potato pulp in piglets

Carsten Jers, Mikael Lenz Strube, Mette D Cantor, Bea K K Nielsen, Ole Brøsted Sørensen, Mette Boye, Anne S. Meyer

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Abstract

We have previously shown that galacto-rhamnogalacturonan fibers can be enzymatically extracted from potato pulp and that these fibers have potential for exerting a prebiotic effect in piglets. The spore-forming Bacillus species are widely used as probiotics in feed supplements for pigs. In this study, we evaluated the option for further functionalizing Bacillus feed supplements by selecting strains possessing the enzymes required for extraction of the potentially prebiotic fibers. We established that it would require production and secretion of pectin lyase and/or polygalacturonase but no or limited secretion of galactanase and β-galactosidase. By screening a library of 158 Bacillus species isolated from feces and soil, we demonstrated that especially strains of Bacillus amyloliquefaciens, Bacillus subtilis, and Bacillus mojavensis have the necessary enzyme profile and thus the capability to degrade polygalacturonan. Using an in vitro porcine gastrointestinal model system, we revealed that specifically strains of B. mojavensis were able to efficiently release galacto-rhamnogalacturonan from potato pulp under simulated gastrointestinal conditions. The work thus demonstrated the feasibility of producing prebiotic fibers via a feed containing Bacillus spores and potato pulp and identified candidates for future in vivo evaluation in piglets.
Original languageEnglish
JournalApplied Microbiology and Biotechnology
Volume101
Issue number9
Pages (from-to)3605-3615
ISSN0175-7598
DOIs
Publication statusPublished - 2017

Keywords

  • B. mojavensis
  • Bacillus
  • Piglet
  • Prebiotic
  • Probiotic
  • Rhamnogalacturonan

Cite this

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title = "Selection of Bacillus species for targeted in situ release of prebiotic galacto-rhamnogalacturonan from potato pulp in piglets",
abstract = "We have previously shown that galacto-rhamnogalacturonan fibers can be enzymatically extracted from potato pulp and that these fibers have potential for exerting a prebiotic effect in piglets. The spore-forming Bacillus species are widely used as probiotics in feed supplements for pigs. In this study, we evaluated the option for further functionalizing Bacillus feed supplements by selecting strains possessing the enzymes required for extraction of the potentially prebiotic fibers. We established that it would require production and secretion of pectin lyase and/or polygalacturonase but no or limited secretion of galactanase and β-galactosidase. By screening a library of 158 Bacillus species isolated from feces and soil, we demonstrated that especially strains of Bacillus amyloliquefaciens, Bacillus subtilis, and Bacillus mojavensis have the necessary enzyme profile and thus the capability to degrade polygalacturonan. Using an in vitro porcine gastrointestinal model system, we revealed that specifically strains of B. mojavensis were able to efficiently release galacto-rhamnogalacturonan from potato pulp under simulated gastrointestinal conditions. The work thus demonstrated the feasibility of producing prebiotic fibers via a feed containing Bacillus spores and potato pulp and identified candidates for future in vivo evaluation in piglets.",
keywords = "B. mojavensis, Bacillus, Piglet, Prebiotic, Probiotic, Rhamnogalacturonan",
author = "Carsten Jers and Strube, {Mikael Lenz} and Cantor, {Mette D} and Nielsen, {Bea K K} and S{\o}rensen, {Ole Br{\o}sted} and Mette Boye and Meyer, {Anne S.}",
year = "2017",
doi = "10.1007/s00253-017-8176-x",
language = "English",
volume = "101",
pages = "3605--3615",
journal = "Applied Microbiology and Biotechnology",
issn = "0175-7598",
publisher = "Springer",
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Selection of Bacillus species for targeted in situ release of prebiotic galacto-rhamnogalacturonan from potato pulp in piglets. / Jers, Carsten; Strube, Mikael Lenz; Cantor, Mette D; Nielsen, Bea K K; Sørensen, Ole Brøsted; Boye, Mette; Meyer, Anne S.

In: Applied Microbiology and Biotechnology, Vol. 101, No. 9, 2017, p. 3605-3615.

Research output: Contribution to journalJournal articleResearchpeer-review

TY - JOUR

T1 - Selection of Bacillus species for targeted in situ release of prebiotic galacto-rhamnogalacturonan from potato pulp in piglets

AU - Jers, Carsten

AU - Strube, Mikael Lenz

AU - Cantor, Mette D

AU - Nielsen, Bea K K

AU - Sørensen, Ole Brøsted

AU - Boye, Mette

AU - Meyer, Anne S.

PY - 2017

Y1 - 2017

N2 - We have previously shown that galacto-rhamnogalacturonan fibers can be enzymatically extracted from potato pulp and that these fibers have potential for exerting a prebiotic effect in piglets. The spore-forming Bacillus species are widely used as probiotics in feed supplements for pigs. In this study, we evaluated the option for further functionalizing Bacillus feed supplements by selecting strains possessing the enzymes required for extraction of the potentially prebiotic fibers. We established that it would require production and secretion of pectin lyase and/or polygalacturonase but no or limited secretion of galactanase and β-galactosidase. By screening a library of 158 Bacillus species isolated from feces and soil, we demonstrated that especially strains of Bacillus amyloliquefaciens, Bacillus subtilis, and Bacillus mojavensis have the necessary enzyme profile and thus the capability to degrade polygalacturonan. Using an in vitro porcine gastrointestinal model system, we revealed that specifically strains of B. mojavensis were able to efficiently release galacto-rhamnogalacturonan from potato pulp under simulated gastrointestinal conditions. The work thus demonstrated the feasibility of producing prebiotic fibers via a feed containing Bacillus spores and potato pulp and identified candidates for future in vivo evaluation in piglets.

AB - We have previously shown that galacto-rhamnogalacturonan fibers can be enzymatically extracted from potato pulp and that these fibers have potential for exerting a prebiotic effect in piglets. The spore-forming Bacillus species are widely used as probiotics in feed supplements for pigs. In this study, we evaluated the option for further functionalizing Bacillus feed supplements by selecting strains possessing the enzymes required for extraction of the potentially prebiotic fibers. We established that it would require production and secretion of pectin lyase and/or polygalacturonase but no or limited secretion of galactanase and β-galactosidase. By screening a library of 158 Bacillus species isolated from feces and soil, we demonstrated that especially strains of Bacillus amyloliquefaciens, Bacillus subtilis, and Bacillus mojavensis have the necessary enzyme profile and thus the capability to degrade polygalacturonan. Using an in vitro porcine gastrointestinal model system, we revealed that specifically strains of B. mojavensis were able to efficiently release galacto-rhamnogalacturonan from potato pulp under simulated gastrointestinal conditions. The work thus demonstrated the feasibility of producing prebiotic fibers via a feed containing Bacillus spores and potato pulp and identified candidates for future in vivo evaluation in piglets.

KW - B. mojavensis

KW - Bacillus

KW - Piglet

KW - Prebiotic

KW - Probiotic

KW - Rhamnogalacturonan

U2 - 10.1007/s00253-017-8176-x

DO - 10.1007/s00253-017-8176-x

M3 - Journal article

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SP - 3605

EP - 3615

JO - Applied Microbiology and Biotechnology

JF - Applied Microbiology and Biotechnology

SN - 0175-7598

IS - 9

ER -