Selection and characterization of phage miniantibodies to actins of different origin

Natalie Kostesha, A. G. Laman, A. O. Shepelyakovskaya, I. S. Zaitseva, V. P. Orlov, L. A. Dykman, F. A: Brovko, O. I. Sokolov

Research output: Contribution to journalJournal articleResearchpeer-review

Abstract

Single-chain miniantibodies (scFv’s) to actin were obtained by the phage display method. A naive combinatorial phage display library of murine scFv’s (containing 2·108 independent recombinant clones) was used to select miniantibodies. After three rounds of selection two clones producing miniantibodies to chicken smooth muscle actin with affinity constants of 1.4·107 and 1.2·106 M−1 were chosen. The isolated miniantibodies could specifically detect various plant and animal actins.
Keyword: Dot-blot assay,Affinity selection,Phage display of miniantibodies,,Actin,Single-chain miniantibodies,Colloidal gold
Original languageEnglish
JournalBiochemistry (Moscow)
Volume70
Issue number8
Pages (from-to)1070-1077
ISSN0006-2979
DOIs
Publication statusPublished - 2005
Externally publishedYes

Fingerprint Dive into the research topics of 'Selection and characterization of phage miniantibodies to actins of different origin'. Together they form a unique fingerprint.

Cite this