Selection and characterization of phage miniantibodies to actins of different origin

Natalie Kostesha; A. G. Laman; A. O. Shepelyakovskaya; I. S. Zaitseva; V. P. Orlov; L. A. Dykman; F. A: Brovko; O. I. Sokolov

doi:10.1007/s10541-005-0198-9

Selection and characterization of phage miniantibodies to actins of different origin

Natalie Kostesha, A. G. Laman, A. O. Shepelyakovskaya, I. S. Zaitseva, V. P. Orlov, L. A. Dykman, F. A: Brovko, O. I. Sokolov

Russian Academy of Sciences

Research output: Contribution to journal › Journal article › Research › peer-review

Abstract

Single-chain miniantibodies (scFv’s) to actin were obtained by the phage display method. A naive combinatorial phage display library of murine scFv’s (containing 2·108 independent recombinant clones) was used to select miniantibodies. After three rounds of selection two clones producing miniantibodies to chicken smooth muscle actin with affinity constants of 1.4·107 and 1.2·106 M−1 were chosen. The isolated miniantibodies could specifically detect various plant and animal actins.
Keyword: Dot-blot assay,Affinity selection,Phage display of miniantibodies,,Actin,Single-chain miniantibodies,Colloidal gold

Original language	English
Journal	Biochemistry (Moscow)
Volume	70
Issue number	8
Pages (from-to)	1070-1077
ISSN	0006-2979
DOIs	https://doi.org/10.1007/s10541-005-0198-9
Publication status	Published - 2005
Externally published	Yes

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title = "Selection and characterization of phage miniantibodies to actins of different origin",

abstract = "Single-chain miniantibodies (scFv{\textquoteright}s) to actin were obtained by the phage display method. A naive combinatorial phage display library of murine scFv{\textquoteright}s (containing 2·108 independent recombinant clones) was used to select miniantibodies. After three rounds of selection two clones producing miniantibodies to chicken smooth muscle actin with affinity constants of 1.4·107 and 1.2·106 M−1 were chosen. The isolated miniantibodies could specifically detect various plant and animal actins. Keyword: Dot-blot assay,Affinity selection,Phage display of miniantibodies,,Actin,Single-chain miniantibodies,Colloidal gold",

author = "Natalie Kostesha and Laman, {A. G.} and Shepelyakovskaya, {A. O.} and Zaitseva, {I. S.} and Orlov, {V. P.} and Dykman, {L. A.} and Brovko, {F. A:} and Sokolov, {O. I.}",

year = "2005",

doi = "10.1007/s10541-005-0198-9",

language = "English",

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T1 - Selection and characterization of phage miniantibodies to actins of different origin

AU - Kostesha, Natalie

AU - Laman, A. G.

AU - Shepelyakovskaya, A. O.

AU - Zaitseva, I. S.

AU - Orlov, V. P.

AU - Dykman, L. A.

AU - Brovko, F. A:

AU - Sokolov, O. I.

PY - 2005

Y1 - 2005

N2 - Single-chain miniantibodies (scFv’s) to actin were obtained by the phage display method. A naive combinatorial phage display library of murine scFv’s (containing 2·108 independent recombinant clones) was used to select miniantibodies. After three rounds of selection two clones producing miniantibodies to chicken smooth muscle actin with affinity constants of 1.4·107 and 1.2·106 M−1 were chosen. The isolated miniantibodies could specifically detect various plant and animal actins. Keyword: Dot-blot assay,Affinity selection,Phage display of miniantibodies,,Actin,Single-chain miniantibodies,Colloidal gold

AB - Single-chain miniantibodies (scFv’s) to actin were obtained by the phage display method. A naive combinatorial phage display library of murine scFv’s (containing 2·108 independent recombinant clones) was used to select miniantibodies. After three rounds of selection two clones producing miniantibodies to chicken smooth muscle actin with affinity constants of 1.4·107 and 1.2·106 M−1 were chosen. The isolated miniantibodies could specifically detect various plant and animal actins. Keyword: Dot-blot assay,Affinity selection,Phage display of miniantibodies,,Actin,Single-chain miniantibodies,Colloidal gold

U2 - 10.1007/s10541-005-0198-9

DO - 10.1007/s10541-005-0198-9

M3 - Journal article

SN - 0006-2979

VL - 70

SP - 1070

EP - 1077

JO - Biochemistry (Moscow)

JF - Biochemistry (Moscow)

IS - 8

ER -

Selection and characterization of phage miniantibodies to actins of different origin

Abstract

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