Selecting the Best: Evolutionary Engineering of Chemical Production in Microbes

Research output: Contribution to journalJournal article – Annual report year: 2018Researchpeer-review

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Selecting the Best: Evolutionary Engineering of Chemical Production in Microbes. / Shepelin, Denis; Hansen, Anne Sofie Lærke; Lennen, Rebecca; Luo, Hao; Herrgård, Markus J.

In: Genes, Vol. 9, No. 5, 249, 2018.

Research output: Contribution to journalJournal article – Annual report year: 2018Researchpeer-review

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@article{0ee9dfd83c3245518bdeebedcb5381f1,
title = "Selecting the Best: Evolutionary Engineering of Chemical Production in Microbes",
abstract = "Microbial cell factories have proven to be an economical means of production for many bulk, specialty, and fine chemical products. However, we still lack both a holistic understanding of organism physiology and the ability to predictively tune enzyme activities in vivo, thus slowing down rational engineering of industrially relevant strains. An alternative concept to rational engineering is to use evolution as the driving force to select for desired changes, an approach often described as evolutionary engineering. In evolutionary engineering, in vivo selections for a desired phenotype are combined with either generation of spontaneous mutations or some form of targeted or random mutagenesis. Evolutionary engineering has been used to successfully engineer easily selectable phenotypes, such as utilization of a suboptimal nutrient source or tolerance to inhibitory substrates or products. In this review, we focus primarily on a more challenging problem-the use of evolutionary engineering for improving the production of chemicals in microbes directly. We describe recent developments in evolutionary engineering strategies, in general, and discuss, in detail, case studies where production of a chemical has been successfully achieved through evolutionary engineering by coupling production to cellular growth.",
keywords = "ALE, Bioproduction, Evolutionary engineering, Genetic engineering, Growth coupling, Metabolic engineering",
author = "Denis Shepelin and Hansen, {Anne Sofie L{\ae}rke} and Rebecca Lennen and Hao Luo and Herrg{\aa}rd, {Markus J.}",
note = "This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).",
year = "2018",
doi = "10.3390/genes9050249",
language = "English",
volume = "9",
journal = "Genes",
issn = "2073-4425",
publisher = "M D P I AG",
number = "5",

}

RIS

TY - JOUR

T1 - Selecting the Best: Evolutionary Engineering of Chemical Production in Microbes

AU - Shepelin, Denis

AU - Hansen, Anne Sofie Lærke

AU - Lennen, Rebecca

AU - Luo, Hao

AU - Herrgård, Markus J.

N1 - This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

PY - 2018

Y1 - 2018

N2 - Microbial cell factories have proven to be an economical means of production for many bulk, specialty, and fine chemical products. However, we still lack both a holistic understanding of organism physiology and the ability to predictively tune enzyme activities in vivo, thus slowing down rational engineering of industrially relevant strains. An alternative concept to rational engineering is to use evolution as the driving force to select for desired changes, an approach often described as evolutionary engineering. In evolutionary engineering, in vivo selections for a desired phenotype are combined with either generation of spontaneous mutations or some form of targeted or random mutagenesis. Evolutionary engineering has been used to successfully engineer easily selectable phenotypes, such as utilization of a suboptimal nutrient source or tolerance to inhibitory substrates or products. In this review, we focus primarily on a more challenging problem-the use of evolutionary engineering for improving the production of chemicals in microbes directly. We describe recent developments in evolutionary engineering strategies, in general, and discuss, in detail, case studies where production of a chemical has been successfully achieved through evolutionary engineering by coupling production to cellular growth.

AB - Microbial cell factories have proven to be an economical means of production for many bulk, specialty, and fine chemical products. However, we still lack both a holistic understanding of organism physiology and the ability to predictively tune enzyme activities in vivo, thus slowing down rational engineering of industrially relevant strains. An alternative concept to rational engineering is to use evolution as the driving force to select for desired changes, an approach often described as evolutionary engineering. In evolutionary engineering, in vivo selections for a desired phenotype are combined with either generation of spontaneous mutations or some form of targeted or random mutagenesis. Evolutionary engineering has been used to successfully engineer easily selectable phenotypes, such as utilization of a suboptimal nutrient source or tolerance to inhibitory substrates or products. In this review, we focus primarily on a more challenging problem-the use of evolutionary engineering for improving the production of chemicals in microbes directly. We describe recent developments in evolutionary engineering strategies, in general, and discuss, in detail, case studies where production of a chemical has been successfully achieved through evolutionary engineering by coupling production to cellular growth.

KW - ALE

KW - Bioproduction

KW - Evolutionary engineering

KW - Genetic engineering

KW - Growth coupling

KW - Metabolic engineering

U2 - 10.3390/genes9050249

DO - 10.3390/genes9050249

M3 - Journal article

VL - 9

JO - Genes

JF - Genes

SN - 2073-4425

IS - 5

M1 - 249

ER -