Screening the natural habitat: New peptaibiotics from specimens and pure cultures of the fungicolous fungus Hypocrea pulvinata

C. R. Roehrich, Anita Iversen, T. Degenkolb, W. M. Jaklitsch, H. Voglmayr, Anette Berg, U. Thrane, A. Vilcinskas, Kristian Fog Nielsen, H. Bruecknerf

    Research output: Contribution to journalConference abstract in journalResearchpeer-review

    Abstract

    In order to further investigate the significance of antibiotics for the producing organism(s) in the natural habitat, we screened specimens of the fungicolous fungus Hypocrea pulvinata growing on its natural hosts Piptoporus betulinus and Fomitopsis pinicola1. Using a peptaibiomics approach2, we detected 17-, 18-, 19- (major sequences), and 20-residue peptide sequences in the five specimens
    analyzed by (U)HPLC/HR-ESI-QqTOF-MS. Structures of peptaibiotics found were independently confirmed by analyzing the peptaibiome of pure agar cultures obtained by single-ascospore isolation from the specimens1. Major, 19-residue peptaibols were assigned as deletion sequences of the trichosporins B3 lacking the Ala/Aib residue in position3. Our results corroborate that: i) peptaibiotics are, indeed, biosynthesized in the natural habitat, thus, ii) their membrane-perturbing formation of ion channels may support the parasitic life style of a fungicolous fungus.
    Original languageEnglish
    JournalJournal of Peptide Science
    Volume18
    Issue numberS1
    Pages (from-to)S74-S74
    ISSN1075-2617
    Publication statusPublished - 2012
    Event32nd EPS Meeting - Athens, Greece
    Duration: 2 Sep 20127 Sep 2012

    Conference

    Conference32nd EPS Meeting
    Country/TerritoryGreece
    CityAthens
    Period02/09/201207/09/2012

    Bibliographical note

    Poster presentation

    Keywords

    • BIOCHEMISTRY
    • CHEMISTRY

    Fingerprint

    Dive into the research topics of 'Screening the natural habitat: New peptaibiotics from specimens and pure cultures of the fungicolous fungus Hypocrea pulvinata'. Together they form a unique fingerprint.

    Cite this